Lysionotin attenuates Staphylococcus aureus pathogenicity by inhibiting α-toxin expression
作者: Zihao TengDongxue ShiHuanyu LiuZiying ShenYonghong ZhaWenhua LiXuming DengJianfeng Wang
作者单位: 1Jilin University
2Changchun University of Chinese Medicine
刊名: Applied Microbiology and Biotechnology, 2017, Vol.101 (17), pp.6697-6703
来源数据库: Springer Journal
DOI: 10.1007/s00253-017-8417-z
关键词: Staphylococcus aureusLysionotinΑ-ToxinPneumonia
英文摘要: α-Toxin, one of the best known pore-forming proteins produced by Staphylococcus aureus ( S. aureus ), is a critical virulence factor in multiple infections. The necessity of α-toxin for S. aureus pathogenicity suggests that this toxin is an important target for the development of a potential treatment strategy. In this study, we showed that lysionotin, a natural compound, can inhibit the hemolytic activity of culture supernatants by S. aureus by reducing α-toxin expression. Using real-time PCR analysis, we showed that transcription of hla (the gene encoding α-toxin) and agr (the locus regulating hla ) was significantly inhibited by lysionotin. Lactate dehydrogenase and live/dead assays indicated that lysionotin effectively protected human alveolar epithelial cells against S. aureus , and...
原始语种摘要: α-Toxin, one of the best known pore-forming proteins produced by Staphylococcus aureus ( S. aureus ), is a critical virulence factor in multiple infections. The necessity of α-toxin for S. aureus pathogenicity suggests that this toxin is an important target for the development of a potential treatment strategy. In this study, we showed that lysionotin, a natural compound, can inhibit the hemolytic activity of culture supernatants by S. aureus by reducing α-toxin expression. Using real-time PCR analysis, we showed that transcription of hla (the gene encoding α-toxin) and agr (the locus regulating hla ) was significantly inhibited by lysionotin. Lactate dehydrogenase and live/dead assays indicated that lysionotin effectively protected human alveolar epithelial cells against S. aureus , and...
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影响因子:3.689 (2012)

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关键词翻译
关键词翻译
  • inhibiting 抑制
  • expression 表示
  • toxin 
  • pathogenicity 病原性
  • potential 
  • pneumonia 肺炎
  • against 反对
  • virulence 病毒性
  • inhibited 被禁止
  • treatment 处理