Colonization study of gfp-tagged Achromobacter marplatensis strain in sugar beet
作者: YingWu ShiChun LiHongMei YangTao ZhangYan GaoMin ChuJun ZengQing LinOuTiKuErYuGuo LiXiangdong HuoKai Lou
作者单位: 1Xinjiang Academy of Agricultural Sciences
2Xinjiang Laboratory of Special Environmental Microbiology
3Xinj
刊名: Journal of Microbiology, 2017, Vol.55 (4), pp.267-272
来源数据库: Springer Journal
DOI: 10.1007/s12275-017-6371-1
关键词: Sugar beetEndophytesGreen fluorescent proteinColonization
英文摘要: This study details the introduction of a gfp marker into an endophytic bacterial strain ( Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its colonization of sugar beet ( Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days. gfp-Expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the Achromobacter marplatensis 17:gfp strain in plants 20 days after inoculation, even in the absence...
原始语种摘要: This study details the introduction of a gfp marker into an endophytic bacterial strain ( Achromobacter marplatensis strain 17, isolated from sugar beet) to monitor its colonization of sugar beet ( Beta. vulgaris L.). Stability of the plasmid encoding the gfp was confirmed in vitro for at least 72 h of bacterial growth and after the colonization of tissues, under nonselective conditions. The colonization was observed using fluorescence microscopy and enumeration of culturable endophytes in inoculated sugar beet plants that grew for 10 or 20 days. gfp-Expressing strains were re-isolated from the inner tissues of surface-sterilized roots and stems of inoculated plants, and the survival of the Achromobacter marplatensis 17:gfp strain in plants 20 days after inoculation, even in the absence...
全文获取路径: Springer  (合作)
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影响因子:1.276 (2012)

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关键词翻译
关键词翻译
  • 细菌的 接种过的
  • sugar 
  • strain 应变
  • tagged 示踪的
  • inoculated 接种过的
  • bacterial 接种过的
  • nonselective 不选择的
  • fluorescent 萤光的
  • introduction 导言
  • colonization 集群现象
  • study 学习