Expression of the zebrafish β-defensin 3 mature peptide in Pichia pastoris and its purification and antibacterial activity
作者: Y. TaoC. F. SongW. Li
作者单位: 1Shanghai Ocean University
刊名: Applied Biochemistry and Microbiology, 2017, Vol.53 (6), pp.661-668
来源数据库: Springer Journal
DOI: 10.1134/S0003683817060126
关键词: ZebrafishΒ-defensin 3Mature peptidePichia pastorisRecombinant protein expressionAntibacterial activity
英文摘要: Defensins are abundant in cells and tissues that are involved in host defense against microbial infections. zfDB3 (zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish ( Danio rerio ). Here we focus on mzfDB3 , which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized mzfDB3 gene with a 6×His-tag at the 3′-end was inserted into the pPICZαA expression vector and transformed into Pichia pastoris X-33 cells. The recombinant zebrafish β-defensin 3 mature peptide ( rmzfDB3 ) was induced with 1.0% methanol at 29°C for 72 h and purified by immobilized metal affinity chromatography. MALDI-TOF/TOF analysis confirmed the expected purified product ( rmzfDB3 , 5.9 kDa). Fermentation supernatant, which contained rmzfDB3, showed...
原始语种摘要: Defensins are abundant in cells and tissues that are involved in host defense against microbial infections. zfDB3 (zebrafish β-defensin 3) is one of 3 copies of defensin β-like genes from zebrafish ( Danio rerio ). Here we focus on mzfDB3 , which is the gene encoding for the zebrafish β-defensin 3 mature peptide. A codon-optimized mzfDB3 gene with a 6×His-tag at the 3′-end was inserted into the pPICZαA expression vector and transformed into Pichia pastoris X-33 cells. The recombinant zebrafish β-defensin 3 mature peptide ( rmzfDB3 ) was induced with 1.0% methanol at 29°C for 72 h and purified by immobilized metal affinity chromatography. MALDI-TOF/TOF analysis confirmed the expected purified product ( rmzfDB3 , 5.9 kDa). Fermentation supernatant, which contained rmzfDB3, showed...
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影响因子:0.689 (2012)

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关键词翻译
关键词翻译
  • defensin 抵御素
  • mature 成孰的
  • activity 活度
  • purified 净化的
  • expression 表示
  • antibacterial 抗细菌的
  • abundant 丰富的
  • inserted 嵌入的
  • contained 容纳的
  • confirmed 已确认的