rDNA cytogenetics and some structural variability in an Avena barbata Pott ex Link × A. sativa subsp. nuda (L.) Gillet et Magne amphiploid after 5-azaC treatment
作者: Maria FlorekRomuald Kosina
作者单位: 1University of Wrocław
刊名: Genetic Resources and Crop Evolution, 2017, Vol.64 (7), pp.1723-1741
来源数据库: Springer Journal
DOI: 10.1007/s10722-016-0469-x
关键词: Ag-NORsAvena amphiploidDemethylationEndosperm variationFISHGISHRDNA
英文摘要: The paper reports on the study of DNA methylation patterns in chromosomes of an octoploid selection obtained from an amphiploid Avena barbata × Avena sativa ssp. nuda , their alteration and plant structural changes induced by 5-azacytidine (5-azaC) treatment. Subtelomere/telomere regions and some chromosomes remained heavily methylated after this practise. The increased concentration of 5-azaC strengthened the demethylation process. NOR rDNA loci on five and 5S rDNA doubled loci on three pairs of chromosomes were detected. Some 5S loci were colocalised with NORs. The number of active NORs was increased by one homologous pair after demethylation and this was related to minor loci. The NORs were mainly located on A/D genomes. Patterns of nucleoli fusion varied between main and lateral roots...
原始语种摘要: The paper reports on the study of DNA methylation patterns in chromosomes of an octoploid selection obtained from an amphiploid Avena barbata × Avena sativa ssp. nuda , their alteration and plant structural changes induced by 5-azacytidine (5-azaC) treatment. Subtelomere/telomere regions and some chromosomes remained heavily methylated after this practise. The increased concentration of 5-azaC strengthened the demethylation process. NOR rDNA loci on five and 5S rDNA doubled loci on three pairs of chromosomes were detected. Some 5S loci were colocalised with NORs. The number of active NORs was increased by one homologous pair after demethylation and this was related to minor loci. The NORs were mainly located on A/D genomes. Patterns of nucleoli fusion varied between main and lateral roots...
全文获取路径: Springer  (合作)
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影响因子:1.593 (2012)

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关键词翻译
关键词翻译
  • amphiploid 双倍体
  • octoploid 八倍体
  • aleurone 糊粉
  • structural 构造
  • starchy 淀粉的
  • treatment 处理
  • demethylation 脱甲基
  • endosperm 胚乳
  • roots 麻根
  • germination 发芽