Trypanosoma cruzi specific mRNA amplification by in vitro transcription improves parasite transcriptomics in host-parasite RNA mixtures
作者: Rafael Luis KesslerDaniela Parada PavoniMarco Aurelio KriegerChristian Macagnan Probst
作者单位: 1Functional Genomics Laboratory, Instituto Carlos Chagas, FIOCRUZ
2Instituto Carlos Chagas, FIOCRUZ
刊名: BMC Genomics, 2017, Vol.18 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12864-017-4163-y
关键词: Trypanosoma cruziTrypanosomatidRNA-SeqTranscriptomicsRNA amplificationT7 RNA polymerase
英文摘要: Trypanosomatids are a group of protozoan parasites that includes the etiologic agents of important human illnesses as Chagas disease, sleeping sickness and leishmaniasis. These parasites have a significant distinction from other eukaryotes concerning mRNA structure, since all mature mRNAs have an identical species-specific sequence of 39 nucleotides at the 5′ extremity, named spliced leader (SL). Considering this peculiar aspect of trypanosomatid mRNA, the aim of the present work was to develop a Trypanosoma cruzi specific in vitro transcription (IVT) linear mRNA amplification method in order to improve parasite transcriptomics analyses.
原始语种摘要: Trypanosomatids are a group of protozoan parasites that includes the etiologic agents of important human illnesses as Chagas disease, sleeping sickness and leishmaniasis. These parasites have a significant distinction from other eukaryotes concerning mRNA structure, since all mature mRNAs have an identical species-specific sequence of 39 nucleotides at the 5′ extremity, named spliced leader (SL). Considering this peculiar aspect of trypanosomatid mRNA, the aim of the present work was to develop a Trypanosoma cruzi specific in vitro transcription (IVT) linear mRNA amplification method in order to improve parasite transcriptomics analyses.
全文获取路径: Springer  (合作)
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影响因子:4.397 (2012)

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关键词翻译
关键词翻译
  • parasite 寄生物
  • transcription 地名转写
  • RNA ROYAL NEPAL AIRLINES CORP.
  • amplification 放大
  • specific 
  • leishmaniasis 利什曼病
  • mature 成孰的
  • spliced 加固的
  • identical 一致的
  • polymerase 聚合酶