Colorimetric and visual determination of microRNA via cycling signal amplification using T7 exonuclease
作者: Ye SangYongjie XuLulu XuWei ChengXinmin LiJiangling WuShijia Ding
作者单位: 1Chongqing Medical University
2Guizhou Provincial People’s Hospital
3The First Affiliated Hospital of Chongqing Medical University
刊名: Microchimica Acta, 2017, Vol.184 (7), pp.2465-2471
来源数据库: Springer Journal
DOI: 10.1007/s00604-017-2238-8
关键词: Visual testEnzymatic amplificationDNA/RNA duplexCapture probeHairpin probesG-quadruplexHeminABTS
英文摘要: A sensitive and specific bioassay based on cyclic enzymatic amplification was developed for determination of microRNA (miRNA) by taking advantage of the exodeoxyribonuclease activity of T7 exonuclease (T7 Exo). In the presence of miRNA, DNA/RNA duplexes are formed by hybridization of miRNA and capture probes (Cp). Then, the Cp is digested to release miRNA into the next amplification cycle assisted by T7 Exo. This leads to the digestion of numerous Cp molecules. The broken Cp does no longer hybridize with hairpin probes (Hp) to unveil G-quadruplex DNAzyme (GDNAs). However, in the absence of miRNA, the Hp hybridizes with Cp to unveil GDNAs. The generated GDNAs form assemblies with hemin to form the G-quadruplex/hemin DNAzyme complex which is capable of catalyzing the oxidation of the...
原始语种摘要: A sensitive and specific bioassay based on cyclic enzymatic amplification was developed for determination of microRNA (miRNA) by taking advantage of the exodeoxyribonuclease activity of T7 exonuclease (T7 Exo). In the presence of miRNA, DNA/RNA duplexes are formed by hybridization of miRNA and capture probes (Cp). Then, the Cp is digested to release miRNA into the next amplification cycle assisted by T7 Exo. This leads to the digestion of numerous Cp molecules. The broken Cp does no longer hybridize with hairpin probes (Hp) to unveil G-quadruplex DNAzyme (GDNAs). However, in the absence of miRNA, the Hp hybridizes with Cp to unveil GDNAs. The generated GDNAs form assemblies with hemin to form the G-quadruplex/hemin DNAzyme complex which is capable of catalyzing the oxidation of the...
全文获取路径: Springer  (合作)
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来源刊物:
影响因子:3.434 (2012)

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关键词翻译
关键词翻译
  • exonuclease 核酸外切酶
  • amplification 放大
  • probes 探针
  • quadruplex 四倍
  • determination 测定
  • Hairpin 发夹
  • hybridize 使...杂交
  • duplex 全双工
  • visual 可见
  • numerous 许多的