Visualization of reticulophagy in living cells using an endoplasmic reticulum-targeted p62 mutant
作者: Liang WangLei LiuLingsong QinQingming LuoZhihong Zhang
作者单位: 1Huazhong University of Science and Technology
刊名: Science China Life Sciences, 2017, Vol.60 (4), pp.333-344
来源数据库: Springer Journal
DOI: 10.1007/s11427-015-9037-4
关键词: ReticulophagyProtein aggregatesEndoplasmic reticulum (ER)LC3P62AutophagosomesOmegasomes
英文摘要: Reticulophagy is a type of selective autophagy in which protein aggregate-containing and/or damaged endoplasmic reticulum (ER) fragments are engulfed for lysosomal degradation, which is important for ER homeostasis. Several chemical drugs and mutant proteins that promote protein aggregate formation within the ER lumen can efficiently induce reticulophagy in mammalian cells. However, the exact mechanism and cellular localization of reticulophagy remain unclear. In this report, we took advantage of the self-oligomerization property of p62/SQSTM1, an adaptor for selective autophagy, and developed a novel reticulophagy system based on an ER-targeted p62 mutant to investigate the process of reticulophagy in living cells. LC3 conversion analysis via western blot suggested that p62 mutant...
原始语种摘要: Reticulophagy is a type of selective autophagy in which protein aggregate-containing and/or damaged endoplasmic reticulum (ER) fragments are engulfed for lysosomal degradation, which is important for ER homeostasis. Several chemical drugs and mutant proteins that promote protein aggregate formation within the ER lumen can efficiently induce reticulophagy in mammalian cells. However, the exact mechanism and cellular localization of reticulophagy remain unclear. In this report, we took advantage of the self-oligomerization property of p62/SQSTM1, an adaptor for selective autophagy, and developed a novel reticulophagy system based on an ER-targeted p62 mutant to investigate the process of reticulophagy in living cells. LC3 conversion analysis via western blot suggested that p62 mutant...
全文获取路径: Springer  (合作)
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来源刊物:
影响因子:2.024 (2011)

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关键词翻译
关键词翻译
  • targeted 对准目标的
  • mutant 突变体
  • cells 麻风细胞
  • marker 标试器
  • triggered 触发的
  • reticulum 蜂巢胃
  • protein 蛋白质
  • aggregate 集合体
  • demonstrated 探明的储量
  • investigate