Rescue of a wild-type rabies virus from cloned cDNA and assessment of the proliferative capacity of recombinant viruses
作者: Qin TianYifei WangQiong ZhangJun LuoMingzhu MeiYongwen LuoXiaofeng Guo
作者单位: 1South China Agricultural University
2Key Laboratory of Zoonosis Prevention and Control of Guangdong Province
刊名: Virus Genes, 2017, Vol.53 (4), pp.573-583
来源数据库: Springer Journal
DOI: 10.1007/s11262-017-1458-7
关键词: Rabies virusReverse genetic systemGene substitutionViral proliferation
英文摘要: Reverse genetic systems (RGS) have been widely used for fixed rabies virus (RABV) strains. However, RGS, for wild-type (wt) strains, have been seldom reported despite the value of this approach in defining the biological characteristics of these strains. In this work, we developed a wt RGS using a swine-origin RABV strain (GD-SH-01) for the first time. In order to have a better understanding of the contribution and function of individual gene on viral proliferation for wt RABV isolates, we constructed a full-length cDNA clone of GD-SH-01 and exchanged the single genes encoding RABV protein of a highly attenuated RABV strain HEP-Flury with those of the virulent strain. Analysis of the viral growth kinetics, cell-to-cell spread, and genomic RNA (gRNA) synthesis of the both the rescued and...
原始语种摘要: Reverse genetic systems (RGS) have been widely used for fixed rabies virus (RABV) strains. However, RGS, for wild-type (wt) strains, have been seldom reported despite the value of this approach in defining the biological characteristics of these strains. In this work, we developed a wt RGS using a swine-origin RABV strain (GD-SH-01) for the first time. In order to have a better understanding of the contribution and function of individual gene on viral proliferation for wt RABV isolates, we constructed a full-length cDNA clone of GD-SH-01 and exchanged the single genes encoding RABV protein of a highly attenuated RABV strain HEP-Flury with those of the virulent strain. Analysis of the viral growth kinetics, cell-to-cell spread, and genomic RNA (gRNA) synthesis of the both the rescued and...
全文获取路径: Springer  (合作)
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来源刊物:
影响因子:1.769 (2012)

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关键词翻译
关键词翻译
  • cDNA 互补 DNA
  • virus 病毒
  • recombinant 重组体
  • genetic 遗传的
  • capacity 电容量
  • strain 应变
  • RGS Radio Guidance System
  • defining 定义
  • together 共同
  • genomic 染色体组的