Nitrile-hydrolyzing enzyme from Meyerozyma guilliermondii and its potential in biosynthesis of 3-hydroxypropionic acid
作者: Qiang ZhangJin-Song GongTing-Ting DongTing-Ting LiuHeng LiWen-Fang DouZhen-Ming LuJin-Song ShiZheng-Hong Xu
作者单位: 1Jiangnan University
刊名: Bioprocess and Biosystems Engineering, 2017, Vol.40 (6), pp.901-910
来源数据库: Springer Journal
DOI: 10.1007/s00449-017-1754-6
关键词: 3-Hydroxypropionic acid3-HydroxypropionitrileMeyerozyma guilliermondiiNitrile-hydrolyzing enzyme
英文摘要: 3-Hydroxypropionic acid (3-HP) is an important platform chemical in organic synthesis. Traditionally, 3-HP was produced by chemical methods and fermentation process. In this work, a novel enzymatic method was developed for green synthesis of 3-HP. A yeast strain harboring nitrile-hydrolyzing enzyme was newly isolated from environmental samples using 3-hydroxypropionitrile (3-HPN) as the sole nitrogen source. It was identified to be Meyerozyma guilliermondii CGMCC12935 by sequencing of the 18S ribosomal DNA and internal transcribed spacer, together with analysis of the morphology characteristics. The catalytic properties of M. guilliermondii CGMCC12935 resting cells were determined, and the optimum activity was achieved at 55 °C and pH 7.5. The enzyme showed broad substrate specificity...
原始语种摘要: 3-Hydroxypropionic acid (3-HP) is an important platform chemical in organic synthesis. Traditionally, 3-HP was produced by chemical methods and fermentation process. In this work, a novel enzymatic method was developed for green synthesis of 3-HP. A yeast strain harboring nitrile-hydrolyzing enzyme was newly isolated from environmental samples using 3-hydroxypropionitrile (3-HPN) as the sole nitrogen source. It was identified to be Meyerozyma guilliermondii CGMCC12935 by sequencing of the 18S ribosomal DNA and internal transcribed spacer, together with analysis of the morphology characteristics. The catalytic properties of M. guilliermondii CGMCC12935 resting cells were determined, and the optimum activity was achieved at 55 °C and pH 7.5. The enzyme showed broad substrate specificity...
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影响因子:1.869 (2012)

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关键词翻译
关键词翻译
  • enzyme 
  • hydrolyzing 水解的
  • activity 活度
  • chemical 化学的
  • nitrogen 
  • sequencing 排序
  • biological 生物学的
  • achieved 获得的
  • excess 超量
  • resting 休息的