Purification and properties of a novel quizalofop- p -ethyl-hydrolyzing esterase involved in quizalofop- p -ethyl degradation by Pseudomonas sp. J-2
作者: Hui ZhangMengya LiJie LiGuangli WangYuan Liu
作者单位: 1Huaibei Normal University
刊名: Microbial Cell Factories, 2017, Vol.16 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12934-017-0695-8
关键词: Quizalofop- p -ethylBiodegradationQpeHMetabolic pathwayPseudomonas sp
英文摘要: Quizalofop- p -ethyl (QPE) is a post-emergence herbicide that effectively controls grass weeds and is often detected in the environment. However, the biochemical and molecular mechanisms of QPE degradation in the environment remains unclear. In this study, a highly effective QPE-degrading bacterial strain J-2 was isolated from acclimated activated sludge and identified as a Pseudomonas sp., containing the QPE breakdown metabolite quizalofop acid (QA) identified by Liquid Chromatography-Ion Trap-Mass Spectrometry (LC-IT-MSn) analysis. A novel QPE hydrolase esterase-encoding gene qpeH was cloned from strain J-2 and functionally expressed in Escherichia coli BL21 (DE3). The specific activity of recombinant QpeH was 198.9 ± 2.7 U mg−1 for QPE with K m and K...
原始语种摘要: Quizalofop- p -ethyl (QPE) is a post-emergence herbicide that effectively controls grass weeds and is often detected in the environment. However, the biochemical and molecular mechanisms of QPE degradation in the environment remains unclear. In this study, a highly effective QPE-degrading bacterial strain J-2 was isolated from acclimated activated sludge and identified as a Pseudomonas sp., containing the QPE breakdown metabolite quizalofop acid (QA) identified by Liquid Chromatography-Ion Trap-Mass Spectrometry (LC-IT-MSn) analysis. A novel QPE hydrolase esterase-encoding gene qpeH was cloned from strain J-2 and functionally expressed in Escherichia coli BL21 (DE3). The specific activity of recombinant QpeH was 198.9 ± 2.7 U mg−1 for QPE with K m and K...
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来源刊物:
影响因子:3.306 (2012)

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关键词翻译
关键词翻译
  • esterase 酯酶
  • novel 长篇小说
  • ethyl 乙基
  • involved 涉及
  • degradation 减嚣夷酌
  • recombinant 重组体
  • hydrolyzing 水解的
  • family 
  • strain 应变
  • expressed 榨出的