Diacylglycerol kinase ε localizes to subsurface cisterns of cerebellar Purkinje cells
作者: Yasukazu HozumiHiroki FujiwaraKenya KanekoSatoshi FujiiMatthew K. TophamMasahiko WatanabeKaoru Goto
作者单位: 1Akita University Graduate School of Medicine
2Yamagata University School of Medicine
3University of Utah
4Hokkaido University Graduate School of Medicine
刊名: Cell and Tissue Research, 2017, Vol.368 (3), pp.441-458
来源数据库: Springer Journal
DOI: 10.1007/s00441-017-2579-y
关键词: ImmunohistochemistryInositol 145-trisphosphate receptorKnockout mouseRat brainBehavior
英文摘要: Following activation of Gq protein-coupled receptors, phospholipase C yields a pair of second messengers: diacylglycerol (DG) and inositol 1,4,5-trisphosphate. Diacylglycerol kinase (DGK) phosphorylates DG to produce phosphatidic acid, another second messenger. Of the DGK family, DGKε is the only DGK isoform that exhibits substrate specificity for DG with an arachidonoyl acyl chain at the sn -2 position. Recently, we demonstrated that hydrophobic residues in the N -terminus of DGKε play an important role in targeting the endoplasmic reticulum in transfected cells. However, its cellular expression and subcellular localization in the brain remain elusive. In the present study, we investigate this issue using specific DGKε antibody. DGKε was richly expressed in principal neurons of higher...
原始语种摘要: Following activation of Gq protein-coupled receptors, phospholipase C yields a pair of second messengers: diacylglycerol (DG) and inositol 1,4,5-trisphosphate. Diacylglycerol kinase (DGK) phosphorylates DG to produce phosphatidic acid, another second messenger. Of the DGK family, DGKε is the only DGK isoform that exhibits substrate specificity for DG with an arachidonoyl acyl chain at the sn -2 position. Recently, we demonstrated that hydrophobic residues in the N -terminus of DGKε play an important role in targeting the endoplasmic reticulum in transfected cells. However, its cellular expression and subcellular localization in the brain remain elusive. In the present study, we investigate this issue using specific DGKε antibody. DGKε was richly expressed in principal neurons of higher...
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影响因子:3.677 (2012)

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关键词翻译
关键词翻译
  • cells 麻风细胞
  • kinase 激酶
  • inositol 肌醇
  • Inositol 环己六醇
  • cerebellar 小脑的
  • receptor 接受体
  • knockout 出模器
  • brain 
  • subsurface 次表层的
  • subcellular 亚细胞的