Suitable reference gene for quantitative real-time PCR analysis of gene expression in gonadal tissues of minnow Puntius sophore under high-temperature stress
作者: Arabinda MahantyGopal Krishna PurohitSasmita MohantyNihar Ranjan NayakBimal Prasanna Mohanty
作者单位: 1ICAR- Central Inland Fisheries Research Institute
2KIIT University
3Wayne State University School of Medicine
刊名: BMC Genomics, 2017, Vol.18 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12864-017-3974-1
关键词: Reference genesRT-qPCRReproductive physiologyThermal stressPuntius sophore
英文摘要: High ambient temperature is known to affect fish gonadal development and physiology in a variety of ways depending on the severity and duration of exposure; however, the underlying molecular mechanisms are poorly understood. Gonadal gene expression influence the gonadal development, physiology and the quality of egg/sperm produced in teleosts and the mechanistic understanding of spatio-temporal changes in the gonadal gene expression could be instrumental in controlling the fate of egg/sperm and the quality of seed produced. Real time-quantititative polymerase chain reaction (RT-qCR), is a high throughput, sensitive and reproducible methodology used for understanding gene expression patterns by measuring the relative abundance of mRNA transcripts. However, its accuracy relies upon a...
原始语种摘要: High ambient temperature is known to affect fish gonadal development and physiology in a variety of ways depending on the severity and duration of exposure; however, the underlying molecular mechanisms are poorly understood. Gonadal gene expression influence the gonadal development, physiology and the quality of egg/sperm produced in teleosts and the mechanistic understanding of spatio-temporal changes in the gonadal gene expression could be instrumental in controlling the fate of egg/sperm and the quality of seed produced. Real time-quantititative polymerase chain reaction (RT-qCR), is a high throughput, sensitive and reproducible methodology used for understanding gene expression patterns by measuring the relative abundance of mRNA transcripts. However, its accuracy relies upon a...
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来源刊物:
影响因子:4.397 (2012)

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关键词翻译
关键词翻译
  • expression 表示
  • gonadal 生殖腺的
  • reference 基准电压源
  • minnow 用作钓饵的小鱼
  • temperature 温度
  • analysis 分析
  • eukaryotic 真核的
  • RNA ROYAL NEPAL AIRLINES CORP.
  • glyceraldehyde 甘油醛
  • understanding 理解