Strand-specific RNA sequencing in pig testes identifies developmentally regulated genes and circular RNAs
作者: Maoliang RanBo WengBin ChenMaisheng WuChangqing HeShanwen Zhang
作者单位: 1Hunan Agriculture University
2Hunan Provincial Key Laboratory for Genetic Improvement of Domestic Animal
3Xiangtan Bureau of Animal Husbandry and Veterinary Medicine and Aquatic Product
刊名: Genes & Genomics, 2017, Vol.39 (10), pp.1083-1094
来源数据库: Springer Journal
DOI: 10.1007/s13258-017-0576-x
关键词: Transcriptomic profileCircRNAsDevelopmentalTestisPigRNA-seq
英文摘要: Testicular development and spermatogenesis are strictly regulated with the complex interactions between several cell types in testis. However, the limited availability of genomic and molecular information limits our understanding of this complex physiological process. In this study, we characterized the transcriptome between immature (30-day-old) and mature (180-day-old) pig testes using RNA-seq technology. 24,469 known coding gene transcripts corresponding to 20,566 genes were mapped in these two developmental stages, 3,328 genes were differentially expressed, and numerous novel transcripts and alternative splicing events were also identified. Ten differentially expressed genes were validated by measuring the relative expression using real-time quantitative polymerase chain reaction...
原始语种摘要: Testicular development and spermatogenesis are strictly regulated with the complex interactions between several cell types in testis. However, the limited availability of genomic and molecular information limits our understanding of this complex physiological process. In this study, we characterized the transcriptome between immature (30-day-old) and mature (180-day-old) pig testes using RNA-seq technology. 24,469 known coding gene transcripts corresponding to 20,566 genes were mapped in these two developmental stages, 3,328 genes were differentially expressed, and numerous novel transcripts and alternative splicing events were also identified. Ten differentially expressed genes were validated by measuring the relative expression using real-time quantitative polymerase chain reaction...
全文获取路径: Springer  (合作)
分享到:
来源刊物:
影响因子:0.497 (2012)

×
关键词翻译
关键词翻译
  • sequencing 排序
  • RNA ROYAL NEPAL AIRLINES CORP.
  • mature 成孰的
  • testes 精巢
  • expressed 榨出的
  • development 开发
  • genomic 染色体组的
  • numerous 许多的
  • several 各自
  • relative 相对的