Effect of centrifugal force on the development of articular neocartilage with bovine primary chondrocytes
作者: J. PrittinenJ. YlärinneJ. PilttiS. S. KarhulaL. RieppoS. P. OjanenR. K. KorhonenS. SaarakkalaM. J. LammiC. Qu
作者单位: 1Department of Integrative Medical Biology, Umeå University
2Nordlab Kokkola, Keski-Pohjanmaa Central Hospital Soite
3Research Unit of Medical Imaging, Physics and Technology, Faculty of Medicine, University of Oulu
4Infotech Doctoral Program, University of Oulu
5Medical Research Center Oulu, Oulu University Hospital and University of Oulu
6Department of Applied Physics, University of Eastern Finland
7Department of Diagnostic Radiology, Oulu University Hospital
8School of Public Health, Health Science Center, Key Laboratory of Trace Elements and Endemic Diseases, National Health and Family Planning Commission, Xi’an Jiaotong University
刊名: Cell and Tissue Research, 2019, Vol.375 (3), pp.629-639
来源数据库: Springer Nature Journal
DOI: 10.1007/s00441-018-2938-3
关键词: Centrifugal forceCartilage tissue engineeringPrimary chondrocyteTissue assemblyOsteoarthritis
英文摘要: Abstract(#br)A lot has been invested into understanding how to assemble cartilage tissue in vitro and various designs have been developed to manufacture cartilage tissue with native-like biological properties. So far, no satisfactory design has been presented. Bovine primary chondrocytes are used to self-assemble scaffold-free constructs to investigate whether mechanical loading by centrifugal force would be useful in manufacturing cartilage tissue in vitro. Six million chondrocytes were laid on top of defatted bone disks placed inside an agarose well in 50-ml culture tubes. The constructs were centrifuged once or three times per day for 15 min at a centrifugal force of 771× g for up to 4 weeks. Control samples were cultured under the same conditions without exposure to centrifugation....
全文获取路径: Springer Nature  (合作)
影响因子:3.677 (2012)