Biotransformation of Ginsenosides Re and Rg 1 into Rg 2 and Rh 1 by Thermostable β -Glucosidase from Thermotoga thermarum
作者: Jianjun PeiTao WuTao YaoLinguo ZhaoGang DingZhenzhong WangWei Xiao
作者单位: 1Nanjing Forestry University
2Jiangsu Key Laboratory of Biomass Based Green Fuels and Chemicals
3Jiangsu Kanion Pharmaceutical Co., Ltd.
4State Key Laboratory of New-tech for Chinese Medicine Pharmaceutical Process
刊名: Chemistry of Natural Compounds, 2017, Vol.53 (3), pp.472-477
来源数据库: Springer Journal
DOI: 10.1007/s10600-017-2025-0
关键词: BiotransformationGinsenosidesΒ -glucosidaseThermotoga thermarum
英文摘要: The recombinant thermostable β -glucosidase from Thermotoga thermarum DSM 5069T exhibited high selectivity to catalyze the conversion of ginsenoside Re and Rg1 to the more pharmacologically active minor ginsenoside Rg2 and Rh1, respectively. At a concentration of 1.36 U/mL of the enzyme, a temperature of 85°C, and pH 5.5, 10 g/L ginsenoside Re was transformed into 8.02 g/L Rg2 within 60 min, and 2 g/L ginsenoside Rg1 was transformed into 1.56 g/L Rh1 within 60 min. This paper provides the first report on the production of ginsenoside Rg2 and Rh1 by a highly thermostable β -glucosidase.
原始语种摘要: The recombinant thermostable β -glucosidase from Thermotoga thermarum DSM 5069T exhibited high selectivity to catalyze the conversion of ginsenoside Re and Rg1 to the more pharmacologically active minor ginsenoside Rg2 and Rh1, respectively. At a concentration of 1.36 U/mL of the enzyme, a temperature of 85°C, and pH 5.5, 10 g/L ginsenoside Re was transformed into 8.02 g/L Rg2 within 60 min, and 2 g/L ginsenoside Rg1 was transformed into 1.56 g/L Rh1 within 60 min. This paper provides the first report on the production of ginsenoside Rg2 and Rh1 by a highly thermostable β -glucosidase.
全文获取路径: Springer  (合作)
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影响因子:0.599 (2012)

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关键词翻译
关键词翻译
  • ginsenoside 人参皂甙
  • thermostable 耐热的
  • within 在以内
  • catalyze 催化
  • concentration 浓度
  • glucosidase 葡萄糖苷酶
  • temperature 温度
  • production 生产
  • conversion 转换
  • minor 次要的