Ribosomal subunit protein typing using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification and discrimination of Aspergillus species
作者: Sayaka NakamuraHiroaki SatoReiko TanakaYoko KusuyaHiroki TakahashiTakashi Yaguchi
作者单位: 1National Institute of Advanced Industrial Science and Technology (AIST)
2Chiba University
刊名: BMC Microbiology, 2017, Vol.17 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12866-017-1009-3
关键词: AspergillusRibosomal subunit proteinsMatrix-assisted laser desorption ionization time-of-flight mass spectrometry
英文摘要: Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on simple mass spectral pattern-matching, with no peak assignment and no taxonomic input. We propose here a ribosomal subunit protein (RSP) typing technique using MALDI-TOF MS for the identification and discrimination of Aspergillus species. The results are concluded to be phylogenetic in that they reflect the molecular evolution of housekeeping RSPs.
原始语种摘要: Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on simple mass spectral pattern-matching, with no peak assignment and no taxonomic input. We propose here a ribosomal subunit protein (RSP) typing technique using MALDI-TOF MS for the identification and discrimination of Aspergillus species. The results are concluded to be phylogenetic in that they reflect the molecular evolution of housekeeping RSPs.
全文获取路径: Springer  (合作)
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来源刊物:
影响因子:3.104 (2012)

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关键词翻译
关键词翻译
  • flight 飞行
  • identification 辨认
  • subunit 亚单位
  • ionization 电离化
  • protein 蛋白质
  • desorption 解吸
  • spectrometry 光谱测定
  • assisted 辅助的
  • laser 激光
  • housekeeping 内务操作