Ribosomal subunit protein typing using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) for the identification and discrimination of Aspergillus species
| 作者： | Sayaka Nakamura, Hiroaki Sato, Reiko Tanaka, Yoko Kusuya, Hiroki Takahashi, Takashi Yaguchi |
| 作者单位： |
1National Institute of Advanced Industrial Science and Technology (AIST) 2Chiba University |
| 刊名： | BMC Microbiology, 2017, Vol.17 (1) |
| 来源数据库： | Springer Journal |
| DOI： | 10.1186/s12866-017-1009-3 |
| 关键词： | Aspergillus; Ribosomal subunit proteins; Matrix-assisted laser desorption ionization time-of-flight mass spectrometry; |
| 英文摘要： | Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on simple mass spectral pattern-matching, with no peak assignment and no taxonomic input. We propose here a ribosomal subunit protein (RSP) typing technique using MALDI-TOF MS for the identification and discrimination of Aspergillus species. The results are concluded to be phylogenetic in that they reflect the molecular evolution of housekeeping RSPs. |
| 原始语种摘要： | Accurate identification of Aspergillus species is a very important subject. Mass spectral fingerprinting using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is generally employed for the rapid identification of fungal isolates. However, the results are based on simple mass spectral pattern-matching, with no peak assignment and no taxonomic input. We propose here a ribosomal subunit protein (RSP) typing technique using MALDI-TOF MS for the identification and discrimination of Aspergillus species. The results are concluded to be phylogenetic in that they reflect the molecular evolution of housekeeping RSPs. |
