Quadruply-labeled serum albumin as a biodegradable nanosensor for simultaneous fluorescence imaging of intracellular pH values, oxygen and temperature
作者: Xiao-ai ZhangWei ZhangQi WangJunli WangGuodong RenXu-dong Wang
作者单位: 1Department of Chemistry, Fudan University
2Laboratory of Biophysics, University of Turku
3State Key Laboratory of Genetic Engineering and Department of Biochemistry and Molecular Biology, School of Life Sciences, Fudan University
刊名: Microchimica Acta, 2019, Vol.186 (8), pp.1-11
来源数据库: Springer Journal
DOI: 10.1007/s00604-019-3674-4
关键词: Simultaneous imagingMultiple sensingTriple sensingNanosensorsLuminescenceIntracellular sensingSerum albuminRatiometric pH sensingLifetime imagingOxygen sensing
英文摘要: Abstract(#br)The construction of multiple fluorescent nanosensors for intracellular studies is a challenging task because spectral overlap of indicator probes can lead to cross-talk and mutual interference. This work describes biodegradable nanosensors that can simultaneously measure three intracellular parameters (temperature, pH and oxygen concentration). Bovine serum albumin (BSA) is selected as the scaffold to construct the triple nanosensor by covalent immobilization four fluorophores on BSA. The following luminophores were used: (a) fluorescein as a probe for pH values, (b) a platinum(II) porphyrin complex for oxygen; (c) a europium(III) clathrate complex for temperature, and (d) a rhodamine B as a reference dye. The nanoparticles have a size of 20 nm and show excellent...
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影响因子:3.434 (2012)

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