Optimization of monomethoxy polyethyleneglycol-modified oxalate decarboxylase by response surface methodology
作者: Han LongXingHua CaiHui YangJunBin HeJia WuRiHui Lin
作者单位: 1Guangxi University for Nationalities
刊名: Journal of Biological Physics, 2017, Vol.43 (3), pp.445-459
来源数据库: Springer Journal
DOI: 10.1007/s10867-017-9461-8
关键词: Oxalate decarboxylaseMonomethoxy polyethyleneglycolResponse surface method (RSM)
英文摘要: In order to improve the stability of oxalate decarboxylase (Oxdc), response surface methodology (RSM), based on a four-factor three-level Box-Behnken central composite design was used to optimize the reaction conditions of oxalate decarboxylase (Oxdc) modified with monomethoxy polyethyleneglycol (mPEG5000). Four independent variables such as the ratio of mPEG-aldehyde to Oxdc, reaction time, temperature, and reaction pH were investigated in this work. The structure of modified Oxdc was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared (FTIR) spectroscopy, the stability of the modified Oxdc was also investigated. The optimal conditions were as follows: the mole ratio of mPEG-aldehyde to Oxdc of 1:47.6, time of 13.1 h,...
原始语种摘要: In order to improve the stability of oxalate decarboxylase (Oxdc), response surface methodology (RSM), based on a four-factor three-level Box-Behnken central composite design was used to optimize the reaction conditions of oxalate decarboxylase (Oxdc) modified with monomethoxy polyethyleneglycol (mPEG5000). Four independent variables such as the ratio of mPEG-aldehyde to Oxdc, reaction time, temperature, and reaction pH were investigated in this work. The structure of modified Oxdc was identified by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Fourier transform infrared (FTIR) spectroscopy, the stability of the modified Oxdc was also investigated. The optimal conditions were as follows: the mole ratio of mPEG-aldehyde to Oxdc of 1:47.6, time of 13.1 h,...
全文获取路径: Springer  (合作)
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影响因子:0.951 (2012)

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关键词翻译
关键词翻译
  • modified 改进
  • decarboxylase 脱羧酶
  • oxalate 草酸盐
  • conditions 条件式
  • methodology 方法学
  • ratio 
  • reaction 反酌
  • aldehyde 
  • treatment 处理
  • higher