Engineering rTCA pathway and C4-dicarboxylate transporter for l -malic acid production
作者: Xiulai ChenYuancai WangXiaoxiang DongGuipeng HuLiming Liu
作者单位: 1Jiangnan University
刊名: Applied Microbiology and Biotechnology, 2017, Vol.101 (10), pp.4041-4052
来源数据库: Springer Journal
DOI: 10.1007/s00253-017-8141-8
关键词: LSaccharomyces cerevisiaeReductive TCA pathwayC4-Dicarboxylate transporterPathway optimization
英文摘要: l -Malic acid is an important component of a vast array of food additives, antioxidants, disincrustants, pharmaceuticals, and cosmetics. Here, we presented a pathway optimization strategy and a transporter modification approach to reconstruct the l -malic acid biosynthesis pathway and transport system, respectively. First, pyruvate carboxylase ( pyc ) and malate dehydrogenase ( mdh ) from Aspergillus flavus and Rhizopus oryzae were combinatorially overexpressed to construct the reductive tricarboxylic acid (rTCA) pathway for l -malic acid biosynthesis. Second, the l -malic acid transporter ( Spmae ) from Schizosaccharomyces pombe was engineered by removing the ubiquitination motification to enhance the l -malic acid efflux system. Finally, the l -malic acid pathway was optimized by...
原始语种摘要: l -Malic acid is an important component of a vast array of food additives, antioxidants, disincrustants, pharmaceuticals, and cosmetics. Here, we presented a pathway optimization strategy and a transporter modification approach to reconstruct the l -malic acid biosynthesis pathway and transport system, respectively. First, pyruvate carboxylase ( pyc ) and malate dehydrogenase ( mdh ) from Aspergillus flavus and Rhizopus oryzae were combinatorially overexpressed to construct the reductive tricarboxylic acid (rTCA) pathway for l -malic acid biosynthesis. Second, the l -malic acid transporter ( Spmae ) from Schizosaccharomyces pombe was engineered by removing the ubiquitination motification to enhance the l -malic acid efflux system. Finally, the l -malic acid pathway was optimized by...
全文获取路径: Springer  (合作)
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影响因子:3.689 (2012)

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关键词翻译
关键词翻译
  • malic 苹果的
  • pathway 轨道
  • transporter 运送机
  • biosynthesis 生物合成
  • tricarboxylic 三(个)羧基的
  • expression 表示
  • optimization 最佳化
  • addition 添加
  • pombe 非洲粟酒
  • neutralizing 中和