Genome modification of CXCR4 by Staphylococcus aureus Cas9 renders cells resistance to HIV-1 infection
作者: Qiankun WangShuliang ChenQiaoqiao XiaoZhepeng LiuShuai LiuPanpan HouLi ZhouWei HouWenzhe HoChunmei LiLi WuDeyin Guo
作者单位: 1Wuhan University
2The Ohio State University
3Sun Yat-sen University
刊名: Retrovirology, 2017, Vol.14 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12977-017-0375-0
关键词: CXCR4HIV-1Primary CD4+ T cellsAdeno-associated virusCRISPR/SaCas9
英文摘要: The CRISPR/Cas9 system has been widely used for genome editing in mammalian cells. CXCR4 is a co-receptor for human immunodeficiency virus type 1 (HIV-1) entry, and loss of CXCR4 function can protect cells from CXCR4 (X4)-tropic HIV-1 infection, making CXCR4 an important target for HIV-1 gene therapy. However, the large size of the CRISPR/SpCas9 system presents an obstacle to its efficient delivery into primary CD4+ T cells. Recently, a small Staphylococcus aureus Cas9 (SaCas9) has been developed as a genome editing tool can address this question. Therefore, it provides a promising strategy for HIV-1 gene therapy if it is used to target CXCR4.
原始语种摘要: The CRISPR/Cas9 system has been widely used for genome editing in mammalian cells. CXCR4 is a co-receptor for human immunodeficiency virus type 1 (HIV-1) entry, and loss of CXCR4 function can protect cells from CXCR4 (X4)-tropic HIV-1 infection, making CXCR4 an important target for HIV-1 gene therapy. However, the large size of the CRISPR/SpCas9 system presents an obstacle to its efficient delivery into primary CD4+ T cells. Recently, a small Staphylococcus aureus Cas9 (SaCas9) has been developed as a genome editing tool can address this question. Therefore, it provides a promising strategy for HIV-1 gene therapy if it is used to target CXCR4.
全文获取路径: Springer  (合作)
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影响因子:5.657 (2012)

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关键词翻译
关键词翻译
  • HIV Hydraulic Isolation Valve
  • cells 麻风细胞
  • infection 感染
  • editing 编辑
  • obstacle 障碍物
  • virus 病毒
  • target 目标
  • genome 基因组
  • mammalian 哺乳动物
  • efficient 有用的