Improving Pullulanase Catalysis via Reversible Immobilization on Modified Fe 3 O 4 @Polydopamine Nanoparticles
作者: Jianfeng WangZhongmei LiuZhemin Zhou
作者单位: 1Jiangnan University
2East China University of Technology
刊名: Applied Biochemistry and Biotechnology, 2017, Vol.182 (4), pp.1467-1477
来源数据库: Springer Nature Journal
DOI: 10.1007/s12010-017-2411-x
关键词: AnoxybacillusNanoparticlesPolydopaminePullulanaseReusability
原始语种摘要: To improve the catalysis of pullulanase from Anoxybacillus sp.WB42, Fe3O4@polydopamine nanoparticles (Fe3O4@PDA) were prepared and modified with functional groups for immobilization of pullulanases via covalent binding or ionic adsorption. Immobilized pullulanases had lower thermal stability than that of free pullulanase, whereas their catalysis depended on the surface characteristics of nanoparticles. As for covalent immobilization of pullulanases onto Fe3O4@PDA derivatives, the spacer grafted onto Fe3O4@PDA made the catalytic efficiency of pullulanase increase up to the equivalence of free enzyme but dramatically reduced the pullulanase thermostability. In contrast, pullulanases bounded ionically to...
全文获取路径: Springer Nature  (合作)
影响因子:1.893 (2012)

  • catalytic 催化的
  • catalysis 催化
  • derivatives 派生物
  • catalyst 催化剂
  • pullulanase 支链淀粉酶
  • adsorption 吸附
  • reaction 反酌
  • immobilization 固定
  • nanoparticle 乳酸
  • efficiency 效率