Isolation and identification of human metabolites from a novel anti-tumor candidate drug 5-chlorogenic acid injection by HPLC-HRMS/MS n and HPLC-SPE-NMR
作者: Tiankun RenYanan WangCaihong WangMengtian ZhangWang HuangJiandong JiangWenbin LiJinlan Zhang
作者单位: 1Peking Union Medical College & Chinese Academy of Medical Sciences
2Jiuzhang Biochemical Engineering Science and Technology Development Co., Ltd.
3Capital Medical University
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (30), pp.7035-7048
来源数据库: Springer Journal
DOI: 10.1007/s00216-017-0657-3
关键词: Human urineMetabolites5-Chlorogenic acid (5-CQA)IdentificationHigh-performance liquid chromatography coupled with high-resolution mass and multiple-stage mass spectrometry (HPLC-HRMS/MSn)High-performance liquid chromatography-solid phase extraction-nuclear magnetic resonance (HPLC-SPE-NMR)
英文摘要: A novel anti-tumor candidate drug, 5-chlorogenic acid (5-CQA) injection, was used for the treatment of malignant glioma in clinical trial (phase I) in China. The isolation and identification of the metabolites of 5-CQA injection in humans were investigated in the present study. Urine and feces samples obtained after intramuscular administration of 5-CQA injection to healthy adults have been analyzed by high-performance liquid chromatography coupled with high-resolution mass and multiple-stage mass spectrometry (HPLC-HRMS/MSn). No metabolite was detected in human feces; however, in human urine, a total of six metabolites were identified including isomerized 5-CQA (P1 and P2), hydrolyzed 5-CQA (M1and M2), and methylated 5-CQA (M3 and M4). Among them, M3 and M4 were the main...
原始语种摘要: A novel anti-tumor candidate drug, 5-chlorogenic acid (5-CQA) injection, was used for the treatment of malignant glioma in clinical trial (phase I) in China. The isolation and identification of the metabolites of 5-CQA injection in humans were investigated in the present study. Urine and feces samples obtained after intramuscular administration of 5-CQA injection to healthy adults have been analyzed by high-performance liquid chromatography coupled with high-resolution mass and multiple-stage mass spectrometry (HPLC-HRMS/MSn). No metabolite was detected in human feces; however, in human urine, a total of six metabolites were identified including isomerized 5-CQA (P1 and P2), hydrolyzed 5-CQA (M1and M2), and methylated 5-CQA (M3 and M4). Among them, M3 and M4 were the main...
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影响因子:3.659 (2012)

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关键词翻译
关键词翻译
  • injection 贯入
  • liquid 液体
  • chromatography 色层分离法
  • performance 性能
  • human 人的
  • phase 相位
  • candidate 选择物
  • NMR N-Modular Redundancy
  • extraction 提取
  • glioma 神经胶质瘤