Integrating multi-omics analyses of Nonomuraea dietziae to reveal the role of soybean oil in [(4′-OH)MeLeu] 4 -CsA overproduction
作者: Huanhuan LiuDi HuangLina JinCheng WangShaoxiong LiangJianping Wen
作者单位: 1Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education
2Tianjin University
3Nankai University, TEDA
4Nankai University
刊名: Microbial Cell Factories, 2017, Vol.16 (1)
来源数据库: Springer Journal
DOI: 10.1186/s12934-017-0739-0
关键词: [(4′-OH)MeLeu]4-CsANonomuraea dietziaeSoybean oilProteomicsMetabolomicsCytochrome P450 hydroxylases
英文摘要: Nonomuraea dietziae is a promising microorganism to mediate the region-specific monooxygenation reaction of cyclosporine A (CsA). The main product [(4′-OH)MeLeu]4-CsA possesses high anti-HIV/HCV and hair growth-stimulating activities while avoiding the immunosuppressive effect of CsA. However, the low conversion efficiency restricts the clinical application. In this study, the production of [(4′-OH)MeLeu]4-CsA was greatly improved by 55.6% from 182.8 to 284.4 mg/L when supplementing soybean oil into the production medium, which represented the highest production of [(4′-OH)MeLeu]4-CsA so far.
原始语种摘要: Nonomuraea dietziae is a promising microorganism to mediate the region-specific monooxygenation reaction of cyclosporine A (CsA). The main product [(4′-OH)MeLeu]4-CsA possesses high anti-HIV/HCV and hair growth-stimulating activities while avoiding the immunosuppressive effect of CsA. However, the low conversion efficiency restricts the clinical application. In this study, the production of [(4′-OH)MeLeu]4-CsA was greatly improved by 55.6% from 182.8 to 284.4 mg/L when supplementing soybean oil into the production medium, which represented the highest production of [(4′-OH)MeLeu]4-CsA so far.
全文获取路径: Springer  (合作)
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影响因子:3.306 (2012)

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关键词翻译
关键词翻译
  • overproduction 过度生产
  • soybean 大豆
  • stimulating 促进
  • mediate 
  • reveal 显露
  • reaction 反酌
  • promising 前提
  • improved 改进
  • region 地域
  • efficiency 效率