Metabolic network model guided engineering ethylmalonyl-CoA pathway to improve ascomycin production in Streptomyces hygroscopicus var. ascomyceticus
| 作者： | Junhua Wang, Cheng Wang, Kejing Song, Jianping Wen |
| 作者单位： |
1Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education 2Tianjin University |
| 刊名： | Microbial Cell Factories, 2017, Vol.16 (1) |
| 来源数据库： | Springer Journal |
| DOI： | 10.1186/s12934-017-0787-5 |
| 关键词： | Ascomycin; FK520; Metabolic network model; Ethylmalonyl-CoA pathway; Streptomyces hygroscopicus var. ascomyceticus; |
| 英文摘要： | Ascomycin is a 23-membered polyketide macrolide with high immunosuppressant and antifungal activity. As the lower production in bio-fermentation, global metabolic analysis is required to further explore its biosynthetic network and determine the key limiting steps for rationally engineering. To achieve this goal, an engineering approach guided by a metabolic network model was implemented to better understand ascomycin biosynthesis and improve its production. |
| 原始语种摘要： | Ascomycin is a 23-membered polyketide macrolide with high immunosuppressant and antifungal activity. As the lower production in bio-fermentation, global metabolic analysis is required to further explore its biosynthetic network and determine the key limiting steps for rationally engineering. To achieve this goal, an engineering approach guided by a metabolic network model was implemented to better understand ascomycin biosynthesis and improve its production. |
