作者: | Junhua Wang, Cheng Wang, Kejing Song, Jianping Wen |
作者单位: |
1Key Laboratory of System Bioengineering (Tianjin University), Ministry of Education 2Tianjin University |
刊名: | Microbial Cell Factories, 2017, Vol.16 (1) |
来源数据库: | Springer Journal |
DOI: | 10.1186/s12934-017-0787-5 |
关键词: | Ascomycin; FK520; Metabolic network model; Ethylmalonyl-CoA pathway; Streptomyces hygroscopicus var. ascomyceticus; |
英文摘要: | Ascomycin is a 23-membered polyketide macrolide with high immunosuppressant and antifungal activity. As the lower production in bio-fermentation, global metabolic analysis is required to further explore its biosynthetic network and determine the key limiting steps for rationally engineering. To achieve this goal, an engineering approach guided by a metabolic network model was implemented to better understand ascomycin biosynthesis and improve its production. |
原始语种摘要: | Ascomycin is a 23-membered polyketide macrolide with high immunosuppressant and antifungal activity. As the lower production in bio-fermentation, global metabolic analysis is required to further explore its biosynthetic network and determine the key limiting steps for rationally engineering. To achieve this goal, an engineering approach guided by a metabolic network model was implemented to better understand ascomycin biosynthesis and improve its production. |