Construction of a trivalent candidate Shigella vaccine strain with host-vector balanced-lethal system
作者: Xianliang RuiYongqiang XuXudong WuGuofu SuCuifen Huang
作者单位: 1Institute of Biotechnology
刊名: Science in China Series C: Life Sciences, 1997, Vol.40 (1), pp.52-59
来源数据库: Springer Nature Journal
DOI: 10.1007/BF02879107
关键词: Shigella sppbalanced-lethal systemrecombinant vaccine strainimmune protection
原始语种摘要: Abstract(#br)A trivalent live Shigella vaccine candidate FSD01 against S. flexneri 2a, S. sonnei and S. dysenteriae I was constructed. This candidate strain was based on the S. flexneri 2a vaccine T32. By homologous recombination exchange, the chromosomal asd gene of T32 was site-specifically inactivated, resulting in the strain unable to grow normally in LB broth, while another asd gene of S. mutans was employed to construct an Asd + complementary vector. This combination of asd - host/Asd + vector formed a balanced-lethal expression system in T32 strain. By use of this system, two important protective antigen genes coding for S. sonnei Form I antigen and Shiga toxin B subunit were cloned and expressed in T32, which led to the construction of trivalent candidate vaccine FSD01....
全文获取路径: Springer Nature  (合作)
影响因子:1.61 (2011)

  • Construction 建筑物
  • system 
  • balanced 平[均]衡
  • candidate 选择物
  • lethal 致死
  • vaccine 菌苗
  • strain 应变
  • vector 矢量
  • trivalent 三价的