Increasing drug resistance in human lung cancer cells by mutant-type p53 gene mediated by retrovirus
作者: Zhenqiang GaoZhiping GaoXifu LiuTao Zhang
作者单位: 1Department of Pathology, Beijing Medical University
刊名: Science in China Series C: Life Sciences, 1997, Vol.40 (1), pp.101-106
来源数据库: Springer Nature Journal
DOI: 10.1007/BF02879112
关键词: human lung cancerp53 genegene cloninggene transferdrug resistance
英文摘要: Abstract(#br)Human mutant-type (mt) p53 cDNA was synthesized and cloned from human lung cancer cell line GL containing mt-p53 gene by using polymerase chain reaction (PCR). It was confirmed that the mt-p53 cDNA contained the complete coding sequence of p53 gene but mutated at codon 245 (G→T) and resulted in glycine to cysteine by sequencing analysis. The retroviral vector pD53M of the mt-p53 was constructed and introduced into the drug-sensitive human lung cancer cells GAO in which p53 gene did not mutate. The transfected GAO cells strongly expressed mutant-type p53 protein by immunohistochemistry, showing that pD53M vector could steadily express in GAO cells. The drug resistance to several anticancer agents of GAO cells infected by pD53M increased in varying degrees, with the highest...
全文获取路径: Springer Nature  (合作)
影响因子:1.61 (2011)

  • mutant 突变体
  • resistance 抵抗
  • retrovirus 还原病毒
  • cells 麻风细胞
  • human 人的
  • cancer 癌症