Sequential conformation change and activation of chicken liver dihydrofolate reductase in low concentration of guanidine hydrochloride
作者: Yingxin FanLi ZhuJunmei ZhouChenglu Zou
作者单位: 1State Key Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences
刊名: Science in China Series C: Life Sciences, 1997, Vol.40 (2), pp.202-209
来源数据库: Springer Nature Journal
DOI: 10.1007/BF02882049
关键词: dihydrofolate reductaseguanidine hydrochlorideactivationprotein unfolding
英文摘要: Abstract(#br)The conformation changes of dihydrofolate reductase (DHFR) from chicken liver in guanidine hydrochloride were monitored by protein intrinsic fluorescence, hydrophobic fluorescence probe TNS and limited proteolysis by proteinase K. The kinetics of the enzyme denaturation were also studied and compared with its activity changes. It was indicated by the enhanced fluorescence of 2- p -toluidinylnaphthalene (TNS) that a subtle conformational change of the enzyme in dilute GuHCl parallels GuHCl-induced activation. At GuHCl concentration higher than 0. 75 mol/L, the conformational change can be detected by increased susceptibility of the enzyme to proteinase K, but no significant gross conformational change of the enzyme molecule is observed by intrinsic fluorescence up to a GuHCl...
全文获取路径: Springer Nature  (合作)
影响因子:1.61 (2011)

  • guanidine 
  • concentration 浓度
  • hydrochloride 盐酸盐
  • conformation 构象
  • change 变化
  • reductase 还原酶
  • chicken 小鸡
  • liver 
  • activation 活化