Biological function of modified nucleotides T 54 and Ψ 55 of yeast tRNA Ala
作者: Huaqi JiangYouxin JinDebao Wang
作者单位: 1State Key Laboratory of Molecular Biology, Shanghai Institute of Biochen, Chinese Academy of Sciences
刊名: Science in China Series C: Life Sciences, 1997, Vol.40 (6), pp.568-575
来源数据库: Springer Nature Journal
DOI: 10.1007/BF02882686
关键词: yeast tRNA Alabiological functionmodified nucleotidesT 54 and Ψ 55
英文摘要: Abstract(#br)The 3′ half molecule of yeast tRNA Ala (nucleotides 36–75) was hybridized with a DNA fragment (5′GGAATCGAACC 3′) and the hybrid was then digested with E. coli RNase H (from Boehringer). The enzyme can specifically cleave the 3′ half molecule at the 3′ side of nucleotide Ψ 55 , thus a fragment C 36 -Ψ 55 was prepared. The 3′-terminal T or TΨ of this fragment was removed by one or two cycles of periodate oxidation and β-elimination. The products were fragments C 36 -T 54 and C 36 -G 53 . Three yeast tRNA Ala fragments C 56 -A 76 , U 55 -A 76 (with Ψ 55 replaced by U), U 54 -A 76 (with T 54 Ψ 55 replaced by UU) were synthesized and ligated with three prepared fragments (C 36 -Ψ 55 , C 36 -T 54 and C 36 -G 53 ) respectively by T4 RNA ligase. The products were further ligated with...
全文获取路径: Springer Nature  (合作)
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影响因子:1.61 (2011)

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关键词翻译
关键词翻译
  • yeast 酵母
  • modified 改进
  • tRNA 转移 RNA
  • function 函数