Functional analysis of the ACTGCTGA sequence motif in the human immunodeficiency virus type-1 long terminal repeat promoter
作者: Sabine E. C. KokenJeroen L. B. WamelJan L. M. C. GeelenBen Berkhout
作者单位: 1Department of Virology Academic Medical Center, University of Amsterdam
刊名: Journal of Biomedical Science, 1994, Vol.1 (2), pp.83-92
来源数据库: Springer Nature Journal
DOI: 10.1007/BF02257981
关键词: Transcription regulationNatural HIV-1 LTR variationPromoter motif duplication
英文摘要: Abstract(#br)The ACTGCTGA sequence (CTG motif) is located immediately upstream of the NF-κB enhancer in the human immunodeficiency virus type-1 (HIV-1) long terminal repeat (LTR). We previously reported on the frequent duplication of this motif in HIV-1-infected individuals. In this study we further characterized the role of the CTG element in transcription and its interaction with cellular proteins. We analyzed the biological activity of LTR promoters with dimeric, monomeric or deleted CTG motifs. Our results indicate that LTRs containing the monomeric CTG motif are the most active transcriptional promoters. Furthermore, mutant viruses with dimeric or deleted CTG motif were consistently out-competed by the wild-type virus in co-culture experiments. Gel mobility shift assays were used to...
全文获取路径: Springer Nature  (合作)
影响因子:2.458 (2012)

  • promoter 催化剂
  • human 人的
  • motif 花纹图案
  • terminal 顶端的
  • sequence 次序
  • analysis 分析
  • virus 病毒
  • repeat 中继