Fc γ Receptor Mediated Phagocytosis by Human Neutrophil Cytoplasts
作者: Howard B. FleitBernard P. Lane
作者单位: 1Department of Pathology, State University of New York at Stony Brook
刊名: Inflammation, 1999, Vol.23 (3), pp.253-262
来源数据库: Springer Nature Journal
DOI: 10.1023/A:1020226020062
英文摘要: Abstract(#br)Neutrophils utilize Fc γ Receptors (Fc γ R) to bind and internalize antibody coated cells or immune complexes. We have compared ultrastructurally Fc γ R-mediated phagocytosis by neutrophil cytoplasts (enucleated and granule-free neutrophils) prepared either by treatment with cytochalasin B (CB-cytoplasts) followed by ultracentrifugation or by brief heating at 45°C in suspension followed by ultracentrifugation. The phagocytosis of antibody-coated erythrocytes (EIgG) or the internalization of crosslinked IgG complexes by cytoplasts prepared by brief heating was comparable to that of untreated neutrophils. In contrast, CB-cytoplasts bound but failed to internalize EIgG or crosslinked IgG complexes. Comparison of these two methods for the preparation of neutrophil cytoplasts may...
全文获取路径: Springer Nature  (合作)
影响因子:2.457 (2012)