Measuring the internal concentration of volatile organic compounds in small organisms using micro-QuEChERS coupled to LVI–GC–MS/MS
作者: Denise KurthMartin KraussTobias SchulzeWerner Brack
作者单位: 1UFZ – Helmholtz Centre for Environmental Research
2RWTH Aachen University
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (25), pp.6041-6052
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0532-2
关键词: Micro-QuEChERSMulti-mode inlet (MMI) GC–MS/MSLarge volume injection (LVI)Small volume internal concentrationZebrafish embryo extraction
英文摘要: There is an increasing demand for analytical tools to measure the internal concentrations of xenobiotic pollutants in small organisms. Such tools are required to determine exposure in ecotoxicological studies yet avoid sophisticated clean-up and enrichment techniques or large-scale experimental design. Thus, this paper presents a modified QuEChERS method coupled to gas chromatography tandem mass spectrometry (GC–MS/MS) for small volume organic samples. Ten zebrafish ( Danio rerio ) embryos were exposed to a 46-compound mixture at 10 ng/mL. After 72 h of exposure, they were extracted in 200 μL glass inserts using 70 μL of both acetonitrile and water. Volumes of 50 μL of extract were injected into a GC–MS/MS with a multi-mode inlet. Internal concentrations of zebrafish embryos could be...
原始语种摘要: There is an increasing demand for analytical tools to measure the internal concentrations of xenobiotic pollutants in small organisms. Such tools are required to determine exposure in ecotoxicological studies yet avoid sophisticated clean-up and enrichment techniques or large-scale experimental design. Thus, this paper presents a modified QuEChERS method coupled to gas chromatography tandem mass spectrometry (GC–MS/MS) for small volume organic samples. Ten zebrafish ( Danio rerio ) embryos were exposed to a 46-compound mixture at 10 ng/mL. After 72 h of exposure, they were extracted in 200 μL glass inserts using 70 μL of both acetonitrile and water. Volumes of 50 μL of extract were injected into a GC–MS/MS with a multi-mode inlet. Internal concentrations of zebrafish embryos could be...
全文获取路径: Springer Nature  (合作)
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影响因子:3.659 (2012)

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关键词翻译
关键词翻译
  • concentration 浓度
  • GC Garbage Collection
  • small 小的
  • internal 内部的
  • coupled 耦合
  • exclusively 专用于
  • organic 有机的
  • micro 
  • required 必需
  • volatile 挥发性的