Construction of a highly sensitive signal-on aptasensor based on gold nanoparticles/functionalized silica nanoparticles for selective detection of tryptophan
作者: Ayemeh Bagheri HashkavayiJahan Bakhsh RaoofReza Ojani
作者单位: 1University of Mazandaran
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (27), pp.6429-6438
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0588-z
关键词: TryptophanHeminSignal-on aptasensorFunctionalized silica nanoparticlesGold nanoparticles
英文摘要: In this work, a highly sensitive, low-cost, and label-free aptasensor based on signal-on mechanisms of response was developed by immobilizing the aptamer on gold nanoparticles (AuNPs)/amine-functionalized silica nanoparticle (FSN)/screen-printed electrode (SPE) surface for highly selective electrochemical detection of tryptophan (Trp). The hemin (Hem), which interacted with the guanine bases of the aptamer, worked as a redox indicator to generate a readable electrochemical signal. The changes in the charge transfer resistance have been monitored using the voltammetry and electrochemical impedance spectroscopic (EIS) techniques. The peak current of Hem linearly increased with increasing concentration of Trp, in differential pulse voltammetry, from 0.06 to 250 nM with a detection limit of...
原始语种摘要: In this work, a highly sensitive, low-cost, and label-free aptasensor based on signal-on mechanisms of response was developed by immobilizing the aptamer on gold nanoparticles (AuNPs)/amine-functionalized silica nanoparticle (FSN)/screen-printed electrode (SPE) surface for highly selective electrochemical detection of tryptophan (Trp). The hemin (Hem), which interacted with the guanine bases of the aptamer, worked as a redox indicator to generate a readable electrochemical signal. The changes in the charge transfer resistance have been monitored using the voltammetry and electrochemical impedance spectroscopic (EIS) techniques. The peak current of Hem linearly increased with increasing concentration of Trp, in differential pulse voltammetry, from 0.06 to 250 nM with a detection limit of...
全文获取路径: Springer Nature  (合作)
分享到:
来源刊物:
影响因子:3.659 (2012)

×
关键词翻译
关键词翻译
  • silica 硅土
  • highly 高度地
  • detection 探测
  • tryptophan 色氨酸
  • impedance 阻抗
  • voltammetry 伏安测量法
  • Trp 色氨酸
  • charge 电荷
  • signal 信号
  • sensitive 灵敏的