HLA genotyping by next-generation sequencing of complementary DNA
作者: Hidenobu SegawaYoji KukitaKikuya Kato
作者单位: 1Osaka Medical Center for Cancer and Cardiovascular Diseases
2Laboratory of Medical Genomics, Nara Institute of Science and Technology
刊名: BMC Genomics, 2017, Vol.18 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s12864-017-4300-7
关键词: Next-generation sequencingCDNAHLAMolecular barcode
英文摘要: Genotyping of the human leucocyte antigen (HLA) is indispensable for various medical treatments. However, unambiguous genotyping is technically challenging due to high polymorphism of the corresponding genomic region. Next-generation sequencing is changing the landscape of genotyping. In addition to high throughput of data, its additional advantage is that DNA templates are derived from single molecules, which is a strong merit for the phasing problem. Although most currently developed technologies use genomic DNA, use of cDNA could enable genotyping with reduced costs in data production and analysis. We thus developed an HLA genotyping system based on next-generation sequencing of cDNA.
原始语种摘要: Genotyping of the human leucocyte antigen (HLA) is indispensable for various medical treatments. However, unambiguous genotyping is technically challenging due to high polymorphism of the corresponding genomic region. Next-generation sequencing is changing the landscape of genotyping. In addition to high throughput of data, its additional advantage is that DNA templates are derived from single molecules, which is a strong merit for the phasing problem. Although most currently developed technologies use genomic DNA, use of cDNA could enable genotyping with reduced costs in data production and analysis. We thus developed an HLA genotyping system based on next-generation sequencing of cDNA.
全文获取路径: Springer Nature  (合作)
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影响因子:4.397 (2012)

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关键词翻译
关键词翻译
  • sequencing 排序
  • complementary 补的
  • generation 世代
  • DNA Deoxyribonucleic Acid
  • genomic 染色体组的
  • additional 追加的
  • region 地域
  • unambiguous 无歧义
  • challenging 有希望的
  • merit 功绩