The establishment and characterization of immortal hepatocyte cell lines from a mouse liver injury model
作者: Prabodh RisalBaik Hwan ChoKarl G. SylvesterJae-Chun KimHyoung Tae KimYeon Jun Jeong
作者单位: 1Chonbuk National University Hospital
2Chonbuk National University Medical School
3Stanford University School of Medicine
刊名: In Vitro Cellular & Developmental Biology - Animal, 2011, Vol.47 (8)
来源数据库: Springer Nature Journal
DOI: 10.1007/s11626-011-9445-0
关键词: In vivo primingHepatocyte cell lineMouseImmortal hepatocyte
英文摘要: Abstract(#br)Hepatocytes are an important research tool used for numerous applications. However, a short life span and a limited capacity to replicate in vitro limit the usefulness of primary hepatocyte cultures. We have hypothesized that in vivo priming of hepatocyte could make them more susceptible to growth factors in the medium for continuous proliferation in vitro. Here, a novel approach used to establish hepatocyte cell lines that included hepatocyte priming in vivo prior to culture with a 3,5-diethoxycarbonyl-1,4-dihydrocollidine diet was attempted. The cell line grew in a monolayer while maintaining a granular cytoplasm and a round nucleus. Electron microscopy displayed hepatocyte-like features including mitochondria, glycogen granules, and the presence of bile canaliculi. This...
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  • establishment 确立
  • mouse 小鼠
  • immortal 不朽的
  • hepatocyte 肝细胞
  • liver 
  • lines 线型
  • injury 损害
  • model 模型