Characterization of the specific interaction between the DNA aptamer sgc8c and protein tyrosine kinase-7 receptors at the surface of T-cells by biosensing AFM
作者: Michael LeitnerAlexandra PoturnayovaConstanze LamprechtSabine WeichMaja SnejdarkovaIvana KarpisovaTibor HianikAndreas Ebner
作者单位: 1Johannes Kepler University Linz
2Comenius University
3Slovak Academy of Sciences
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (11), pp.2767-2776
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0238-5
关键词: DNA aptamerPTK7T-cellSingle molecule force spectroscopyEnergy landscapeMolecular recognitionRecognition imaging
英文摘要: We studied the interaction of the specific DNA aptamer sgc8c immobilized at the AFM tip with its corresponding receptor, the protein tyrosine kinase-7 (PTK7) embedded in the membrane of acute lymphoblastic leukemia (ALL) cells (Jurkat T-cells). Performing single molecule force spectroscopy (SMFS) experiments, we showed that the aptamer sgc8c bound with high probability (38.3 ± 7.48%) and high specificity to PTK7, as demonstrated by receptor blocking experiments and through comparison with the binding behavior of a nonspecific aptamer. The determined kinetic off-rate (koff = 5.16 s−1) indicates low dissociation of the sgc8c–PTK7 complex. In addition to the pulling force experiments, simultaneous topography and recognition imaging (TREC) experiments using AFM tips...
原始语种摘要: We studied the interaction of the specific DNA aptamer sgc8c immobilized at the AFM tip with its corresponding receptor, the protein tyrosine kinase-7 (PTK7) embedded in the membrane of acute lymphoblastic leukemia (ALL) cells (Jurkat T-cells). Performing single molecule force spectroscopy (SMFS) experiments, we showed that the aptamer sgc8c bound with high probability (38.3 ± 7.48%) and high specificity to PTK7, as demonstrated by receptor blocking experiments and through comparison with the binding behavior of a nonspecific aptamer. The determined kinetic off-rate (koff = 5.16 s−1) indicates low dissociation of the sgc8c–PTK7 complex. In addition to the pulling force experiments, simultaneous topography and recognition imaging (TREC) experiments using AFM tips...
全文获取路径: Springer Nature  (合作)
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影响因子:3.659 (2012)

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关键词翻译
关键词翻译
  • recognition 识别
  • AFM Amplitude-Frequency Modulation
  • Recognition 识别
  • cells 麻风细胞
  • imaging 图像形成
  • surface 
  • simultaneous 同时的
  • protein 蛋白质
  • specific 
  • interaction 相互酌