Determination of proflavine in rat whole blood without sample pretreatment by laser desorption postionization mass spectrometry
作者: Jiaxin ChenYongjun HuQiao LuPengchao WangHuaqi Zhan
作者单位: 1South China Normal University
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (11), pp.2813-2819
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0225-x
关键词: ProflavineWhole bloodPretreatment-freeDrug levelLaser desorption postionizationMass spectrometry
英文摘要: A novel pretreatment-free method involving laser desorption postionization (LDPI) coupled with time-of-flight mass spectrometry (MS) was developed for the monitoring of proflavine level in rat whole blood. It comprises a protocol for dosing via intravenous administration and collection of whole blood, followed by direct LDPI-MS analysis without any sample pretreatment. An intense ion signal at m / z 209 was observed from whole blood without any interference signals, except some background signals below m / z 100. The calibration curve was established with use of 9-phenylacridine as the internal standard for proflavine determination from the plotting of the peak ratios of proflavine to the internal standard, with a correlation coefficient ( R 2) greater than 0.99. The limit of...
原始语种摘要: A novel pretreatment-free method involving laser desorption postionization (LDPI) coupled with time-of-flight mass spectrometry (MS) was developed for the monitoring of proflavine level in rat whole blood. It comprises a protocol for dosing via intravenous administration and collection of whole blood, followed by direct LDPI-MS analysis without any sample pretreatment. An intense ion signal at m / z 209 was observed from whole blood without any interference signals, except some background signals below m / z 100. The calibration curve was established with use of 9-phenylacridine as the internal standard for proflavine determination from the plotting of the peak ratios of proflavine to the internal standard, with a correlation coefficient ( R 2) greater than 0.99. The limit of...
全文获取路径: Springer Nature  (合作)
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影响因子:3.659 (2012)

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关键词翻译
关键词翻译
  • proflavine 前黄素
  • desorption 解吸
  • whole 全部的
  • pretreatment 预处理
  • blood 血液
  • spectrometry 光谱测定
  • laser 激光
  • dosing 配量
  • ionization 电离化
  • flight 飞行