Super-sensitive time-resolved fluoroimmunoassay for thyroid-stimulating hormone utilizing europium(III) nanoparticle labels achieved by protein corona stabilization, short binding time, and serum preprocessing
作者: Tuomas NäreojaJessica M. RosenholmUrpo LamminmäkiPekka E. Hänninen
作者单位: 1University of Turku
2Karolinska Universitetssjukhuset
3Åbo akademi University
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (13), pp.3407-3416
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0284-z
关键词: Sandwich-type immunoassayTime-resolved fluoroimmunoassayNanoparticle bioconjugateImmunoassay interferenceNanoparticle protein corona
英文摘要: Thyrotropin or thyroid-stimulating hormone (TSH) is used as a marker for thyroid function. More precise and more sensitive immunoassays are needed to facilitate continuous monitoring of thyroid dysfunctions and to assess the efficacy of the selected therapy and dosage of medication. Moreover, most thyroid diseases are autoimmune diseases making TSH assays very prone to immunoassay interferences due to autoantibodies in the sample matrix. We have developed a super-sensitive TSH immunoassay utilizing nanoparticle labels with a detection limit of 60 nU L−1 in preprocessed serum samples by reducing nonspecific binding. The developed preprocessing step by affinity purification removed interfering compounds and improved the recovery of spiked TSH from serum. The sensitivity...
原始语种摘要: Thyrotropin or thyroid-stimulating hormone (TSH) is used as a marker for thyroid function. More precise and more sensitive immunoassays are needed to facilitate continuous monitoring of thyroid dysfunctions and to assess the efficacy of the selected therapy and dosage of medication. Moreover, most thyroid diseases are autoimmune diseases making TSH assays very prone to immunoassay interferences due to autoantibodies in the sample matrix. We have developed a super-sensitive TSH immunoassay utilizing nanoparticle labels with a detection limit of 60 nU L−1 in preprocessed serum samples by reducing nonspecific binding. The developed preprocessing step by affinity purification removed interfering compounds and improved the recovery of spiked TSH from serum. The sensitivity...
全文获取路径: Springer Nature  (合作)
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影响因子:3.659 (2012)

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关键词翻译
关键词翻译
  • immunoassay 免疫测定法
  • protein 蛋白质
  • stabilization 稳定
  • stimulating 促进
  • thyroid 甲状腺
  • nanoparticle 乳酸
  • preprocessing 预处理
  • binding 装订
  • resolved 分辨
  • TSH Track Safety Handbook