Sol–gel encapsulation of pullulanase in the presence of hybrid magnetic (Fe 3 O 4 –chitosan) nanoparticles improves thermal and operational stability
作者: Jie LongXingfei LiXiaobei ZhanXueming XuYaoqi TianZhengjun XieZhengyu Jin
作者单位: 1Jiangnan University
刊名: Bioprocess and Biosystems Engineering, 2017, Vol.40 (6), pp.821-831
来源数据库: Springer Nature Journal
DOI: 10.1007/s00449-017-1747-5
关键词: ImmobilizationPullulanaseSol–gel encapsulationStabilityMagnetic chitosan/Fe3O4 nanoparticle
英文摘要: Pullulanase was sol–gel encapsulated in the presence of magnetic chitosan/Fe3O4 nanoparticles. The resulting immobilized pullulanase was characterized by scanning electron microscopy, vibrating sample magnetometry, Fourier transform infrared spectroscopy and thermogravimetric analysis. The results showed that the addition of pullulanase created a more regular surface on the sol–gel matrix and an enhanced magnetic response to an applied magnetic field. The maximal activity retention (83.9%) and specific activity (291.7 U/mg) of the immobilized pullulanase were observed under optimized conditions including an octyltriethoxysilane:tetraethoxysilane (OTES:TEOS) ratio of 1:2 and enzyme concentration of 0.484 mg/mL sol. The immobilized enzyme exhibited good thermal...
原始语种摘要: Pullulanase was sol–gel encapsulated in the presence of magnetic chitosan/Fe3O4 nanoparticles. The resulting immobilized pullulanase was characterized by scanning electron microscopy, vibrating sample magnetometry, Fourier transform infrared spectroscopy and thermogravimetric analysis. The results showed that the addition of pullulanase created a more regular surface on the sol–gel matrix and an enhanced magnetic response to an applied magnetic field. The maximal activity retention (83.9%) and specific activity (291.7 U/mg) of the immobilized pullulanase were observed under optimized conditions including an octyltriethoxysilane:tetraethoxysilane (OTES:TEOS) ratio of 1:2 and enzyme concentration of 0.484 mg/mL sol. The immobilized enzyme exhibited good thermal...
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影响因子:1.869 (2012)

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关键词翻译
关键词翻译
  • encapsulation 密封
  • chitosan 壳聚糖
  • pullulanase 支链淀粉酶
  • thermal 热的
  • crosslinking 交联
  • nanoparticle 乳酸
  • encapsulated 压缩
  • hybrid 杂种
  • magnetic 磁的
  • enzyme