Development of an Agrobacterium -Mediated Transformation Method and Evaluation of Two Exogenous Constitutive Promoters in Oleaginous Yeast Lipomyces starkeyi
作者: Xinping LinSasa LiuRuiqi BaoNing GaoSufang ZhangRongqian ZhuZongbao Kent Zhao
作者单位: 1National Engineering Research Center of Seafood
2Dalian Institute of Chemical Physics
刊名: Applied Biochemistry and Biotechnology, 2017, Vol.183 (3), pp.867-875
来源数据库: Springer Nature Journal
DOI: 10.1007/s12010-017-2469-5
关键词: Lipomyces starkeyiAgrobacterium -mediated transformationPromotersGenetic engineeringBiofuels
英文摘要: Oleaginous yeast Lipomyces starkeyi , a promising strain of great biotechnical importance, is able to accumulate over 60% of its cell biomass as triacylglycerols (TAGs). It is promising to directly produce the derivatives of TAGs, such as long-chain fatty acid methyl esters and alkanes, in L. starkeyi . However, techniques for genetic modification of this oleaginous yeast are lacking, thus, further research is needed to develop genetic tools and functional elements. Here, we used two exogenous promoters (pGPD and pPGK) from oleaginous yeast Rhodosporidium toruloides to establish a simpler Agrobacterium -mediated transformation (AMT) method for L. starkeyi . Hygromycin-resistant transformants were obtained on antibiotic-contained plate. Mitotic stability test, genotype verification by PCR,...
原始语种摘要: Oleaginous yeast Lipomyces starkeyi , a promising strain of great biotechnical importance, is able to accumulate over 60% of its cell biomass as triacylglycerols (TAGs). It is promising to directly produce the derivatives of TAGs, such as long-chain fatty acid methyl esters and alkanes, in L. starkeyi . However, techniques for genetic modification of this oleaginous yeast are lacking, thus, further research is needed to develop genetic tools and functional elements. Here, we used two exogenous promoters (pGPD and pPGK) from oleaginous yeast Rhodosporidium toruloides to establish a simpler Agrobacterium -mediated transformation (AMT) method for L. starkeyi . Hygromycin-resistant transformants were obtained on antibiotic-contained plate. Mitotic stability test, genotype verification by PCR,...
全文获取路径: Springer Nature  (合作)
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影响因子:1.893 (2012)

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关键词翻译
关键词翻译
  • oleaginous 含油的
  • transformation 变换
  • genetic 遗传的
  • produce 制作
  • hygromycin 潮霉素
  • expression 表示
  • contained 容纳的
  • functional 功能的
  • demonstrated 探明的储量
  • promising 前提