Development of Dof (DNA binding with one finger) transcription factor gene-specific primers through data mining as a functional marker and their use for genetic diversity study in barley ( Hordeum vulgare L.) germplasm
作者: Sahar RouhianDaryoush Nabati AhmadiKarim Sorkheh
作者单位: 1Shahid Chamran University of Ahvaz
刊名: Genes & Genomics, 2017, Vol.39 (5), pp.567-579
来源数据库: Springer Nature Journal
DOI: 10.1007/s13258-016-0510-7
关键词: DNA-binding with one zinc finger (Dof)Domain proteinsHomologHordeum vulgare L.Genetic structure and diversityTranscription factor
英文摘要: The genetic diversity among Hordeum vulgare L. species were assessed based on PCR amplification pattern derived from 75 set of Dof domain and Dof genes specific primers. Multiple bands showing variability in terms of both number and sizes of bands ranging from 0.1 to 3.0 kbp were observed. Out of a total of 2449 bands, 2328 polymorphic and 121 monomorphic bands were obtained and the percentage of polymorphism ranged from 70.27 to 100%. A very high degree of polymorphism was observed with all the primers except HvDof3, HvDof4, HvDof10, HvDof16, HvDof18, HvDof18, HvDof24, Dof4, Dof11, Dof13, Dof15, Dof16, Dof19, Dof20, Dof21, Dof22, Dof23, Dof28, dof38, sbDof23 and sbDof24 primers. Unweighted pair group method based on arithmetic average (UPGMA) analysis was performed on Jaccard’s...
原始语种摘要: The genetic diversity among Hordeum vulgare L. species were assessed based on PCR amplification pattern derived from 75 set of Dof domain and Dof genes specific primers. Multiple bands showing variability in terms of both number and sizes of bands ranging from 0.1 to 3.0 kbp were observed. Out of a total of 2449 bands, 2328 polymorphic and 121 monomorphic bands were obtained and the percentage of polymorphism ranged from 70.27 to 100%. A very high degree of polymorphism was observed with all the primers except HvDof3, HvDof4, HvDof10, HvDof16, HvDof18, HvDof18, HvDof24, Dof4, Dof11, Dof13, Dof15, Dof16, Dof19, Dof20, Dof21, Dof22, Dof23, Dof28, dof38, sbDof23 and sbDof24 primers. Unweighted pair group method based on arithmetic average (UPGMA) analysis was performed on Jaccard’s...
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影响因子:0.497 (2012)

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关键词翻译
关键词翻译
  • genetic 遗传的
  • germplasm 种质
  • functional 功能的
  • binding 装订
  • marker 标试器
  • specific 
  • observed 观察到的
  • finger 梳形物
  • factor 因素
  • barley 大麦