Characterization and in Vitro Inhibition Studies of Bacillus anthracis FtsZ: A Potential Antibacterial Target
作者: Hae-Chul ParkVinayakumar GediJune-Haeng ChoJae-Wook HyunKwang-Jick LeeJeongWoo KangByungJae SoMoon-Young Yoon
作者单位: 1Department of Chemistry and Institute of Natural Science, Hanyang University
2Veterinary Drugs & Biologics Division, Animal and Plant Quarantine Agency (QIA)
3Citrus Research Station, National Institute of Horticultural and Herbal Science, RDA
刊名: Applied Biochemistry and Biotechnology, 2014, Vol.172 (6), pp.3263-3270
来源数据库: Springer Nature Journal
DOI: 10.1007/s12010-014-0752-2
关键词: Antianthrax agentBacillus anthracisCell divisionFtsZNaturally extracted chemicalsScreening
英文摘要: Abstract(#br)FtsZ is an essential bacterial cell division protein that is an attractive target for the development of antibacterial agents. FtsZ is a homologue of eukaryotic tubulin, has GTPase activity, and forms a ring-type structure to initiate cell division. In this study, the FtsZ of Bacillus anthracis was cloned into a bacterial expression vector and overexpressed into Escherichia coli BL21 (DE3) cells. The overexpressed B. anthracis FtsZ was soluble and purified to homogeneity using Ni-His-tag affinity chromatography. Like other known FtsZs, the recombinant B. anthracis FtsZ also showed GTP-dependent polymerization, which was analyzed using both spectrophotometric and Transmission Electronic Microscopic (TEM) analysis. Using the purified FtsZ, we screened a naturally extracted...
全文获取路径: Springer Nature  (合作)
影响因子:1.893 (2012)