Screening of promoters from Arthrobacter sp. CGMCC 3584 using a green fluorescent protein reporter system
作者: Huanqing NiuWei YangKun ZhuangXiaochun ChenYong ChenDong LiuJinglan WuChenjie ZhuHanjie Ying
作者单位: 1Nanjing Tech University
2National Engineering Technique Research Center for Biotechnology
3Jiangsu National Synergetic Innovation Center for Advanced Materials (SICAM)
4National Engineering Technique Research Center for
刊名: World Journal of Microbiology and Biotechnology, 2017, Vol.33 (11)
来源数据库: Springer Nature Journal
DOI: 10.1007/s11274-017-2375-6
关键词: ArthrobacterGreen fluorescent proteinPromoter screeningDeletion analysis
英文摘要: Available molecular and genetic tools for the genetic manipulation of Arthrobacter species are limited until now. In gene engineering, a continuous set of promoters with various strengths are of importance for fine-tuning gene expression in metabolic optimization and control analysis. Here, for the first time, we constructed a promoter trap system using green fluorescence protein (GFP) as a reporter, for screening and characterizing functional Arthrobacter promoters. Twenty-three Arthrobacter transformants of various GFP fluorescence strengths were isolated and characterized through the analysis of DNA sequences. Among the 23 putative promoters, 2 were selected for deletion analysis of promoter elements. As a result, the deletion of the upstream of the putative promoter P8 and P13 caused...
原始语种摘要: Available molecular and genetic tools for the genetic manipulation of Arthrobacter species are limited until now. In gene engineering, a continuous set of promoters with various strengths are of importance for fine-tuning gene expression in metabolic optimization and control analysis. Here, for the first time, we constructed a promoter trap system using green fluorescence protein (GFP) as a reporter, for screening and characterizing functional Arthrobacter promoters. Twenty-three Arthrobacter transformants of various GFP fluorescence strengths were isolated and characterized through the analysis of DNA sequences. Among the 23 putative promoters, 2 were selected for deletion analysis of promoter elements. As a result, the deletion of the upstream of the putative promoter P8 and P13 caused...
全文获取路径: Springer Nature  (合作)
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关键词翻译
关键词翻译
  • fluorescent 萤光的
  • reporter 报告生成程序
  • protein 蛋白质
  • screening 筛分
  • promoter 催化剂
  • genetic 遗传的
  • functional 功能的
  • together 共同
  • upstream 向上游
  • analysis 分析