A silver bullet in a golden age of functional genomics: the impact of Agrobacterium -mediated transformation of fungi
作者: Alexander IdnurmAndy M. BaileyTimothy C. CairnsCandace E. ElliottGary D. FosterGiuseppe IaniriJunhyun Jeon
作者单位: 1University of Melbourne
2University of Bristol
3Technische Universität Berlin
4Duke University Medical Center
5Yeungnam University
刊名: Fungal Biology and Biotechnology, 2017, Vol.4 (1)
来源数据库: Springer Nature Journal
DOI: 10.1186/s40694-017-0035-0
关键词: Functional genomicsMycotaPathogenicity genesRhizobium radiobacterTransfer DNA
英文摘要: The implementation of Agrobacterium tumefaciens as a transformation tool revolutionized approaches to discover and understand gene functions in a large number of fungal species. A. tumefaciens mediated transformation ( At MT) is one of the most transformative technologies for research on fungi developed in the last 20 years, a development arguably only surpassed by the impact of genomics. At MT has been widely applied in forward genetics, whereby generation of strain libraries using random T-DNA insertional mutagenesis, combined with phenotypic screening, has enabled the genetic basis of many processes to be elucidated. Alternatively, At MT has been fundamental for reverse genetics, where mutant isolates are generated with targeted gene deletions or disruptions, enabling gene functional...
原始语种摘要: The implementation of Agrobacterium tumefaciens as a transformation tool revolutionized approaches to discover and understand gene functions in a large number of fungal species. A. tumefaciens mediated transformation ( At MT) is one of the most transformative technologies for research on fungi developed in the last 20 years, a development arguably only surpassed by the impact of genomics. At MT has been widely applied in forward genetics, whereby generation of strain libraries using random T-DNA insertional mutagenesis, combined with phenotypic screening, has enabled the genetic basis of many processes to be elucidated. Alternatively, At MT has been fundamental for reverse genetics, where mutant isolates are generated with targeted gene deletions or disruptions, enabling gene functional...
全文获取路径: Springer Nature  (合作)
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关键词翻译
关键词翻译
  • transformation 变换
  • functional 功能的
  • genetic 遗传的
  • fungi 真菌
  • eukaryotic 真核的
  • impact 冲击碰撞
  • ascomycetes 子囊菌
  • mutagenesis 突变形成
  • demonstrated 探明的储量
  • random 随机的