New approach for cystic fibrosis diagnosis based on chloride/potassium ratio analyzed in non-invasively obtained skin-wipe sweat samples by capillary electrophoresis with contactless conductometric detection
作者: Pavol ĎurčFrantišek ForetEva PokojováLukáš HomolaJana SkřičkováVladimír HeroutMilan DastychHana VinohradskáPetr Kubáň
作者单位: 1CEITEC Masaryk University
2Masaryk University
3University Hospital Brno
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (14), pp.3507-3514
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0318-6
关键词: Cystic fibrosisCapillary electrophoresisSweatSkin wipeIon ratioDiagnosis
原始语种摘要: A new approach for sweat analysis used in cystic fibrosis (CF) diagnosis is proposed. It consists of a noninvasive skin-wipe sampling followed by analysis of target ions using capillary electrophoresis with contactless conductometric detection (C4D). The skin-wipe sampling consists of wiping a defined skin area with precleaned cotton swab moistened with 100 μL deionized water. The skin-wipe sample is then extracted for 3 min into 400 μL deionized water, and the extract is analyzed directly. The developed sampling method is cheap, simple, fast, and painless, and can replace the conventional pilocarpine-induced sweat chloride test commonly applied in CF diagnosis. The aqueous extract of the skin-wipe sample content is analyzed simultaneously by capillary electrophoresis with contactless...
全文获取路径: Springer Nature  (合作)
影响因子:3.659 (2012)

  • conductometric 测量导热率的
  • ratio 
  • capillary 毛状的
  • sweat 
  • contactless 无接点的
  • chloride 氯化物
  • fibrosis 纤维化
  • concentrations 浓聚物
  • cystic 膀胱的
  • values 价值观