Identification and characterization of a biosynthetic gene cluster for tryptophan dimers in deep sea-derived Streptomyces sp. SCSIO 03032
作者: Liang MaWenjun ZhangYiguang ZhuGuangtao ZhangHaibo ZhangQingbo ZhangLiping ZhangChengshan YuanChangsheng Zhang
作者单位: 1Chinese Academy of Sciences
2University of the Chinese Academy of Sciences
刊名: Applied Microbiology and Biotechnology, 2017, Vol.101 (15), pp.6123-6136
来源数据库: Springer Nature Journal
DOI: 10.1007/s00253-017-8375-5
关键词: Tryptophan dimersSpiroindimicinsGene clusterBiosynthesisHeterologous expressionDeep sea
英文摘要: Tryptophan dimers (TDs) are an important class of natural products with diverse bioactivities and share conserved biosynthetic pathways. We report the identification of a partial gene cluster ( spm ) responsible for the biosynthesis of a class of unusual TDs with non-planar skeletons including spiroindimicins (SPMs), indimicins (IDMs), and lynamicins (LNMs) from the deep-sea derived Streptomyces sp. SCSIO 03032. Bioinformatics analysis, targeted gene disruptions, and heterologous expression studies confirmed the involvement of the spm gene cluster in the biosynthesis of SPM/IDM/LNMs, and revealed the indispensable roles for the halogenase/reductase pair SpmHF, the amino acid oxidase SpmO, and the chromopyrrolic acid (CPA) synthase SpmD, as well as the positive regulator SpmR and the...
原始语种摘要: Tryptophan dimers (TDs) are an important class of natural products with diverse bioactivities and share conserved biosynthetic pathways. We report the identification of a partial gene cluster ( spm ) responsible for the biosynthesis of a class of unusual TDs with non-planar skeletons including spiroindimicins (SPMs), indimicins (IDMs), and lynamicins (LNMs) from the deep-sea derived Streptomyces sp. SCSIO 03032. Bioinformatics analysis, targeted gene disruptions, and heterologous expression studies confirmed the involvement of the spm gene cluster in the biosynthesis of SPM/IDM/LNMs, and revealed the indispensable roles for the halogenase/reductase pair SpmHF, the amino acid oxidase SpmO, and the chromopyrrolic acid (CPA) synthase SpmD, as well as the positive regulator SpmR and the...
全文获取路径: Springer Nature  (合作)
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影响因子:3.689 (2012)

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关键词翻译
关键词翻译
  • biosynthetic 生物合成
  • cluster 
  • biosynthesis 生物合成
  • heterologous 异种的
  • expression 表示
  • several 各自
  • confirmed 已确认的
  • monooxygenase 加一氧酶
  • SPM 太阳质子监视器
  • targeted 对准目标的