A new metabolomics-based strategy for identification of endogenous markers of urine adulteration attempts exemplified for potassium nitrite
作者: Andrea E. SteuerKim ArnoldTom D. SchneiderMichael PoetzschThomas Kraemer
作者单位: 1University of Zurich
刊名: Analytical and Bioanalytical Chemistry, 2017, Vol.409 (26), pp.6235-6244
来源数据库: Springer Nature Journal
DOI: 10.1007/s00216-017-0567-4
关键词: Urine adulterationBiomarker identificationMetabolomics methodsHigh-resolution mass spectrometry
英文摘要: Urine adulteration to circumvent positive drug testing represents a problem for toxicological laboratories. While creatinine is a suitable marker for dilution, detection of chemicals is often performed by dipstick tests associated with high rates of false positives. Several methods would be necessary to check for all possible adulterants. Untargeted mass spectrometry (MS) methods used in metabolomics should theoretically allow detecting concentration changes of any endogenous urinary metabolite or presence of new biomarkers produced by chemical adulteration. As a proof of concept study, urine samples from 10 volunteers were treated with KNO2 and analyzed by high-resolution MS. For statistical data evaluation, XCMSplus and MetaboAnalyst were used. Compound...
原始语种摘要: Urine adulteration to circumvent positive drug testing represents a problem for toxicological laboratories. While creatinine is a suitable marker for dilution, detection of chemicals is often performed by dipstick tests associated with high rates of false positives. Several methods would be necessary to check for all possible adulterants. Untargeted mass spectrometry (MS) methods used in metabolomics should theoretically allow detecting concentration changes of any endogenous urinary metabolite or presence of new biomarkers produced by chemical adulteration. As a proof of concept study, urine samples from 10 volunteers were treated with KNO2 and analyzed by high-resolution MS. For statistical data evaluation, XCMSplus and MetaboAnalyst were used. Compound...
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影响因子:3.659 (2012)

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关键词翻译
关键词翻译
  • adulteration 掺杂
  • identification 辨认
  • spectrometry 光谱测定
  • urine 尿
  • endogenous 内成的
  • markers 时标
  • database 资料库
  • routine 程序
  • strategy 战略
  • concentration 浓度