A Simple Nano-SiO 2 -Based ELISA Method for Residue Detection of 2,4-Dichlorophenoxyacetic Acid in Bean Sprouts
作者: Qian-Long WangJie LiXing-De LiLi-Sheng DingJing XieLin-Sen Qing
作者单位: 1Chengdu Institute of Biology, Chinese Academy of Sciences
2University of Chinese Academy of Sciences
3Ohio State University
4Chengdu Medical College
刊名: Food Analytical Methods, 2017, Vol.10 (5), pp.1500-1506
来源数据库: Springer Nature Journal
DOI: 10.1007/s12161-016-0709-x
关键词: 24-Dichlorophenoxyacetic acidResidue detectionSilicon dioxide nanoparticleDirect competitive ELISABean sprout
英文摘要: The abusive use of 2,4-dichlorophenoxyacetic acid (2,4-D) was very common in the commercial production of bean sprouts. Herein, a simple and highly sensitive nano-SiO2-based ELISA method for residual 2,4-D detection was developed. The test depends on the direct competitive combination to anti-2,4-D antibody labeled by horseradish peroxidase (HRP) between nano-SiO2 immobilized by ovalbumin-2,4-D (SiO2-OVA-2,4-D) and free 2,4-D in sample. The linear range of this method was from 1 to 350 ng/mL ( r = 0.997), and the limit of detection (LOD) was 0.079 ng/mL. The coefficient variations of intra-day and inter-day were all lower than 10% at three concentrations (20, 150, and 250 ng/mL). The mean recovery was 93.2%, and the specificity was satisfactory. The...
原始语种摘要: The abusive use of 2,4-dichlorophenoxyacetic acid (2,4-D) was very common in the commercial production of bean sprouts. Herein, a simple and highly sensitive nano-SiO2-based ELISA method for residual 2,4-D detection was developed. The test depends on the direct competitive combination to anti-2,4-D antibody labeled by horseradish peroxidase (HRP) between nano-SiO2 immobilized by ovalbumin-2,4-D (SiO2-OVA-2,4-D) and free 2,4-D in sample. The linear range of this method was from 1 to 350 ng/mL ( r = 0.997), and the limit of detection (LOD) was 0.079 ng/mL. The coefficient variations of intra-day and inter-day were all lower than 10% at three concentrations (20, 150, and 250 ng/mL). The mean recovery was 93.2%, and the specificity was satisfactory. The...
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影响因子:1.969 (2012)

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关键词翻译
关键词翻译
  • ELISA 酶联免疫吸收分析
  • antibody 抗体
  • ovalbumin 卵白蛋白
  • determination 测定
  • dioxide 二氧化物
  • sprout 发芽儿
  • peroxidase 过氧物酶
  • horseradish 辣根
  • labeled 示踪的
  • detection 探测