An Indirect Competitive Immunoassay for Analysis of Carminic Acid in Meat Products
作者: Fan YangHuan WangJuan YangYinpu YueMeiqiang KeHuayuan LiDingyi WanFengrong He
作者单位: 1Wuhan Cloud-Clone Co., Ltd
刊名: Food Analytical Methods, 2017, Vol.10 (11), pp.3687-3693
来源数据库: Springer Nature Journal
DOI: 10.1007/s12161-017-0947-6
关键词: Carminic acidFood additiveMeat productsPolyclonal antibodyIc-ELISAHPLC
原始语种摘要: Carminic acid (CA), a member of natural dyes, is well known for its good stability in a variety of foods such as candies, drinks, and fruits. However, it has never been authorized as a food additive in meat products. In order to detect CA with high sensitivity and specificity, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) has been developed. A high-titer polyclonal antibody (Pab) against CA was obtained after the immunization of New Zealand white rabbits with conjugate of CA coupling with BSA. Several physicochemical factors including pH value, ionic strength, and organic solvent were tested, and it was observed that the ELISA working solution was pH 7.4 PBS buffer containing 50 mmol L−1 Na+. Under the optimal conditions, 50% inhibitive...
全文获取路径: Springer Nature  (合作)
影响因子:1.969 (2012)

  • antibody 抗体
  • immunization 免疫法
  • titer 滴定率
  • ELISA 酶联免疫吸收分析
  • immunosorbent 免疫吸收剂
  • indirect 间接的
  • polyclonal 多元性繁殂系的
  • inhibitive 压制
  • demonstrated 探明的储量
  • spiked 有齿的