Detection of staphylococcal enterotoxin a by phage display mediated immuno-PCR method
作者: A. A. ArtykovK. K. FursovaD. Yu. RyazantsevM. P. ShchannikovaI. V. LoskutovaA. O. ShepelyakovskayaA. G. LamanS. K. ZavrievF. A. Brovko
作者单位: 1Russian Academy of Sciences
2Ernst All-Russia Research Institute of Animal Husbandry
刊名: Russian Journal of Bioorganic Chemistry, 2017, Vol.43 (5), pp.540-543
来源数据库: Springer Nature Journal
DOI: 10.1134/S1068162017050028
关键词: Staphylococcal enterotoxin APhage antibodiesImmuno-PCRDetection in milk
英文摘要: A method of staphylococcal enterotoxin A (SEA) detection in pasteurized milk using recombinant miniantibodies exposed on phage particles was developed. Miniantibodies bind to the antigen, while phage DNA serves as a matrix in polymerase chain reaction (PCR). The SEA detection limit in milk by a phage display mediated immuno-PCR method is 100 pg/mL that is much lower than the level of toxic and allergic dose.
原始语种摘要: A method of staphylococcal enterotoxin A (SEA) detection in pasteurized milk using recombinant miniantibodies exposed on phage particles was developed. Miniantibodies bind to the antigen, while phage DNA serves as a matrix in polymerase chain reaction (PCR). The SEA detection limit in milk by a phage display mediated immuno-PCR method is 100 pg/mL that is much lower than the level of toxic and allergic dose.
全文获取路径: Springer Nature  (合作)
分享到:
来源刊物:
影响因子:0.523 (2012)

×
关键词翻译
关键词翻译
  • enterotoxin 肠毒素
  • phage 噬菌体
  • immuno 免疫
  • staphylococcal 葡萄球菌引起的
  • PCR Page Control Register
  • display 显示
  • recombinant 重组体
  • allergic 变应性的
  • antigen 抗原
  • polymerase 聚合酶