Proteome analysis of circulating exosomes in health and breast cancer
作者: S. N. TamkovichY. S. BakakinaO. S. TutanovA. K. SomovN. A. KirushinaL. V. DubovskayaI. D. VolotovskiP. P. Laktionov
作者单位: 1Russian Academy of Sciences
2Moscow State University
3Novosibirsk State University
4National Academy of Sciences of Belarus
5Novosibirsk Regional Clinical Oncology Health Center
6Novosibirsk Research Institute of Blood Circulation Pathology
刊名: Russian Journal of Bioorganic Chemistry, 2017, Vol.43 (2), pp.126-134
来源数据库: Springer Nature Journal
DOI: 10.1134/S1068162017020157
关键词: ExosomesBloodBreast cancerProteomicsTwo-dimensional gel electrophoresisFlow cytometryProteinsBiomarkers
英文摘要: Microvesicles were isolated from blood plasma and total blood of healthy females and breast cancer patients by filtration and ultracentrifugation. According to flow cytometry, different subpopulations of exosomes were represented in blood of healthy donors and cancer patients at different levels with median fluorescence intensity (MFI) values in both groups arranged in the following order: CD24/СD9 > СD9/СD81 > CD9/CD63 = CD24/CD63. Concentration of exosomes in blood plasma of healthy females estimated by nanoparticle tracking analysis (NTA) did not exceed (3.71 ± 1.15) × 107 particles/mL of blood and did not differ from that in plasma of breast cancer patients, which averaged (3.99 ± 1.03) × 107 particles/mL of blood. Concentration of total exosomes in blood...
原始语种摘要: Microvesicles were isolated from blood plasma and total blood of healthy females and breast cancer patients by filtration and ultracentrifugation. According to flow cytometry, different subpopulations of exosomes were represented in blood of healthy donors and cancer patients at different levels with median fluorescence intensity (MFI) values in both groups arranged in the following order: CD24/СD9 > СD9/СD81 > CD9/CD63 = CD24/CD63. Concentration of exosomes in blood plasma of healthy females estimated by nanoparticle tracking analysis (NTA) did not exceed (3.71 ± 1.15) × 107 particles/mL of blood and did not differ from that in plasma of breast cancer patients, which averaged (3.99 ± 1.03) × 107 particles/mL of blood. Concentration of total exosomes in blood...
全文获取路径: Springer Nature  (合作)
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影响因子:0.523 (2012)

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关键词翻译
关键词翻译
  • breast 胸部
  • differ 不同
  • cancer 癌症
  • blood 血液
  • analysis 分析
  • diagnostics 诊断学
  • absence 缺席
  • isolation 隔离
  • plasma 深绿玉髓
  • identification 辨认